Jin Ziqing, Dang Ling, Li Yan, Feng Chen, Song Xinling, Wei Zhihui, Liu Jie, Wang Hao, Zhang Yichan
Food Science College of Shanxi Normal University, Taiyuan, China.
Shanxi Technology and Business University, Taiyuan, China.
Front Nutr. 2025 Apr 4;12:1571161. doi: 10.3389/fnut.2025.1571161. eCollection 2025.
is a traditional homologous material of medicine and food, but data on its multifunctional peptides are little.
In this study, glutelin-2 was hydrolyzed by alcalase and trypsin assisted with ultrasound. Antihypertensive and antioxidant peptides with ferrous-binding activity were isolated, identified, and screened from the hydrolysates, and the action mechanisms against Keap1 and angiotensin-I-converting enzyme (ACE), gastrointestinal stability, and ferrous-binding capacity were studied.
After Sephadex G-15 isolation, electrospray ionization mass spectrometry, and AHTpin and Peptide Ranker database screening, a safe multifunctional octapeptide: Pro-Val-Asp-Phe-Ala-Gly-Phe-Tyr (PVDFAGFY), was obtained. The capacities of PVDFAGFY to restrain ACE, chelate ferrous ions, and quench hydroxyl radical were IC:105.61 μmol/L, 11.67 mg/g, and 97.67%, respectively. PVDFAGFY restrained ACE via competitively linking to its catalytic (His383) and/or crucial binding sites (Gln281, Lys511, Tyr523, Tyr520, or Ala354), and it can inhibit the Keap1-Nrf2 interaction by binding to 6 residues of Keap1. Ferrous ions were primarily chelated by -hydroxyl, carboxyl, and/or amino groups of PVDFAGFY via ionic forces. Gastrointestinal hydrolysis did not decrease the capacity of PVDFAGFY to antioxidant and restrain ACE ( > 0.05). The ACE inhibition model and activity of PVDFAGFY were not altered by iron chelation; however, PVDFAGFY-ferrous chelate showed lower hydroxyl and ABTS radical quenching capacity and ferric reducing ability than PVDFAGFY ( < 0.05). The gastrointestinal stability and transmembrane absorption of ferrous ions were increased by PVDFAGFY ( < 0.05). Thus, PVDFAGFY may be exploited as ingredients of hypotensive, antioxidant, and/or iron supplementary agents, but antioxidant and hypotensive efficiencies need further study.
是一种传统的药食同源物质，但其多功能肽的数据较少。
本研究中，谷蛋白-2在碱性蛋白酶和胰蛋白酶的作用下，辅以超声进行水解。从水解产物中分离、鉴定并筛选出具有亚铁结合活性的降压和抗氧化肽，并研究其对Keap1和血管紧张素I转换酶（ACE）的作用机制、胃肠道稳定性和亚铁结合能力。
经葡聚糖凝胶G-15分离、电喷雾电离质谱以及AHTpin和肽排名数据库筛选，得到一种安全的多功能八肽：Pro-Val-Asp-Phe-Ala-Gly-Phe-Tyr（PVDFAGFY）。PVDFAGFY抑制ACE、螯合亚铁离子和淬灭羟自由基的能力分别为IC：105.61μmol/L、11.67mg/g和97.67%。PVDFAGFY通过竞争性地与ACE的催化位点（His383）和/或关键结合位点（Gln281、Lys511、Tyr523、Tyr520或Ala354）结合来抑制ACE，并且它可以通过与Keap1的6个残基结合来抑制Keap1-Nrf2相互作用。亚铁离子主要通过离子力与PVDFAGFY的羟基、羧基和/或氨基螯合。胃肠道水解并未降低PVDFAGFY的抗氧化和抑制ACE的能力（>0.05）。铁螯合未改变PVDFAGFY的ACE抑制模型和活性；然而·，PVDFAGFY-亚铁螯合物的羟自由基和ABTS自由基淬灭能力以及铁还原能力低于PVDFAGFY（<0.05）。PVDFAGFY提高了亚铁离子的胃肠道稳定性和跨膜吸收（<0.05）。因此，PVDFAGFY可作为降压、抗氧化和/或补铁剂的成分加以开发，但抗氧化和降压效率有待进一步研究。
