Interdisciplinary Institute of NMR and Molecular Sciences, Key Laboratory of Coal Conversion and New Carbon Materials of Hubei Province, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology, Wuhan 430081, China.
Key Laboratory for Special Functional Materials of Ministry of Education, School of Materials Science and Engineering, Collaborative Innovation Center of Nano Functional Materials and Applications, Henan University, Zhengzhou 450046, China.
Proc Natl Acad Sci U S A. 2024 Apr 2;121(14):e2318391121. doi: 10.1073/pnas.2318391121. Epub 2024 Mar 25.
The exploitation of novel wound healing methods with real-time infection sensing and high spatiotemporal precision is highly important for human health. Pt-based metal-organic cycles/cages (MOCs) have been employed as multifunctional antibacterial agents due to their superior Pt-related therapeutic efficiency, various functional subunits and specific geometries. However, how to rationally apply these nanoscale MOCs on the macroscale with controllable therapeutic output is still challenging. Here, a centimeter-scale Pt MOC film was constructed via multistage assembly and subsequently coated on a N,N'-dimethylated dipyridinium thiazolo[5,4-d]thiazole (MPT)-stained silk fabric to form a smart wound dressing for bacterial sensing and wound healing. The MPT on silk fabric could be used to monitor wound infection in real-time through the bacteria-mediated reduction of MPT to its radical form via a color change. The MPT radical also exhibited an excellent photothermal effect under 660 nm light irradiation, which could not only be applied for photothermal therapy but also induce the disassembly of the Pt MOC film suprastructure. The highly ordered Pt MOC film suprastructure exhibited high biosafety, while it also showed improved antibacterial efficiency after thermally induced disassembly. In vitro and in vivo studies revealed that the combination of the Pt MOC film and MPT-stained silk can provide real-time information on wound infection for timely treatment through noninvasive techniques. This study paves the way for bacterial sensing and wound healing with centimeter-scale metal-organic materials.
开发具有实时感染感应和高时空精度的新型伤口愈合方法对于人类健康至关重要。基于 Pt 的金属有机环/笼 (MOC) 由于其优越的与 Pt 相关的治疗效率、各种功能亚基和特定几何形状而被用作多功能抗菌剂。然而，如何合理地将这些纳米级 MOC 应用于具有可控治疗效果的宏观尺度仍然具有挑战性。在这里，通过多级组装构建了厘米级 Pt MOC 薄膜，然后将其涂覆在 N,N'-二甲化二吡啶并噻唑[5,4-d]噻唑 (MPT) 染色的丝织物上，形成用于细菌感应和伤口愈合的智能伤口敷料。丝织物上的 MPT 可以通过细菌介导的 MPT 还原为其自由基形式来实时监测伤口感染，通过颜色变化。MPT 自由基在 660nm 光照射下也表现出极好的光热效应，不仅可用于光热治疗，还可诱导 Pt MOC 薄膜超结构的分解。高度有序的 Pt MOC 薄膜超结构表现出很高的生物安全性，而在热诱导分解后也显示出提高的抗菌效率。体外和体内研究表明，Pt MOC 薄膜和 MPT 染色丝的组合可以通过非侵入性技术提供伤口感染的实时信息，以便及时进行治疗。这项研究为使用厘米级金属有机材料进行细菌感应和伤口愈合铺平了道路。
