Department of Biomedical Engineering, National Institute of Technology, Raipur 492010, India.
Columbia Institute of Pharmacy, Raipur, India.
Biomed Mater. 2022 Apr 20;17(3). doi: 10.1088/1748-605X/ac64dd.
An ideal wound dressing material should enhance the wound healing process and must avoid bacterial contamination. In this study, the synergistic effect of graphene oxide (GO), silver (Ag) and magnesium (Mg) based silk electrospun nanofibrous film on wound healing was evaluated. It reports the influence of essential elements Mg and Ag during the skin regeneration process. Silver and magnesium nanoparticles were doped in graphene oxide. The goal of the present study was to fabricate an electrospun nanofibrous patch with nanoscale fillers to improve the wound recuperation manner and decrease the recuperation time to forestall microorganism infections and improve cellular behavior. Doping was done to insert Agand Mgions in the crystal lattice of GO to overcome the disadvantage of aggregation of Ag and Mg nanoparticles. In this study, Mgand Agions doped GO functionalized silk fibroin/PVA dressing material was prepared using the electrospinning technique. It was found that, Mg-GO@NSF/PVA and Ag/Mg-GO@NSF/PVA film possess good cytocompatibility, low hemolytic effect and effective antibacterial and anti-biofilm activities. Furthermore, their improved hydrophilicity and mid-range water vapor transmission rate allow them to be a suitable wound dressing material. Tensile strength of the composite silk film were enhanced relatively to silk/PVA film. The effect of prepared film on wound repair were investigated in excision rat model. It indicates, the wound covered with Ag/Mg-GO@NSF/PVA film showed the highest wound contraction rate and re-epithelization, allowing faster repair of wound sites. In conclusion, the development of metallic ions doped GO based silk fibroin/PVA is a promising approach towards development of antibiotic free wound dressing material. It prevents anti-biofilm formation and also provides adequate therapeutic effects for accelerating wound healing.
一种理想的创伤敷料材料应能促进伤口愈合过程，并能避免细菌污染。本研究评估了氧化石墨烯（GO）、银（Ag）和镁（Mg）基丝电纺纳米纤维膜对伤口愈合的协同作用。报告了在皮肤再生过程中必需元素 Mg 和 Ag 的影响。将银和镁纳米颗粒掺杂到氧化石墨烯中。本研究的目的是制备具有纳米级填充物的电纺纳米纤维贴片，以改善伤口恢复方式，缩短恢复时间，防止微生物感染，改善细胞行为。掺杂是为了将 Ag 和 Mg 离子插入 GO 的晶格中，以克服 Ag 和 Mg 纳米颗粒聚集的缺点。在这项研究中，采用静电纺丝技术制备了 Mg 和 Ag 掺杂 GO 功能化丝素/PVA 敷料材料。结果发现，Mg-GO@NSF/PVA 和 Ag/Mg-GO@NSF/PVA 薄膜具有良好的细胞相容性、低溶血效应以及有效的抗菌和抗生物膜活性。此外，它们提高的亲水性和中程水蒸气传输率使它们成为一种合适的创伤敷料材料。与丝素/PVA 薄膜相比，复合丝膜的拉伸强度得到了提高。在切除大鼠模型中研究了制备膜对伤口修复的影响。结果表明，用 Ag/Mg-GO@NSF/PVA 膜覆盖的伤口具有最高的伤口收缩率和再上皮化，使伤口部位更快修复。总之，开发金属离子掺杂 GO 基丝素/PVA 是一种有前途的无抗生素创伤敷料材料的方法。它可以防止生物膜的形成，并为加速伤口愈合提供足够的治疗效果。
