Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
Fish Shellfish Immunol. 2024 Jun;149:109524. doi: 10.1016/j.fsi.2024.109524. Epub 2024 Mar 23.
Recent studies have increasingly linked miRNAs with the modulation of inflammatory responses and immunosuppressive activities. This investigation reveals that mir-30e-3p selectively binds to and modulates gimap8, as demonstrated by luciferase reporter assays and qPCR analyses. Upon LPS stimulation of CIK cells, mir-30e-3p expression was notably elevated, inversely correlating with a decrease in gimap8 mRNA levels. Overexpression of mir-30e-3p attenuated the mRNA levels of pro-inflammatory cytokines beyond the effect of LPS alone, suggesting a regulatory role of mir-30e-3p in inflammation mediated by the gimap8 gene. These insights contribute to our understanding of the complex mechanisms governing inflammatory and immune responses.
最近的研究越来越多地将 miRNAs 与炎症反应的调节和免疫抑制活性联系起来。这项研究表明，mir-30e-3p 选择性地结合并调节 gimap8，这一点通过荧光素酶报告基因检测和 qPCR 分析得到了证实。在 CIK 细胞受到 LPS 刺激后，mir-30e-3p 的表达显著升高，与 gimap8 mRNA 水平的下降呈负相关。过表达 mir-30e-3p 可以减弱 LPS 单独作用后的促炎细胞因子的 mRNA 水平，表明 mir-30e-3p 在 gimap8 基因介导的炎症中具有调节作用。这些发现有助于我们理解调节炎症和免疫反应的复杂机制。
