miRNA-30e-3p 通过靶向草鱼肾脏（CIK）细胞中的 gimap8 基因来调节炎症反应。
MicroRNA-30e-3p regulates the inflammatory response by targeting the gimap8 gene in Ctenopharyngodon idella kidney (CIK) cells.
机构信息
Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China.
Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
出版信息
Fish Shellfish Immunol. 2024 Jun;149:109524. doi: 10.1016/j.fsi.2024.109524. Epub 2024 Mar 23.
Recent studies have increasingly linked miRNAs with the modulation of inflammatory responses and immunosuppressive activities. This investigation reveals that mir-30e-3p selectively binds to and modulates gimap8, as demonstrated by luciferase reporter assays and qPCR analyses. Upon LPS stimulation of CIK cells, mir-30e-3p expression was notably elevated, inversely correlating with a decrease in gimap8 mRNA levels. Overexpression of mir-30e-3p attenuated the mRNA levels of pro-inflammatory cytokines beyond the effect of LPS alone, suggesting a regulatory role of mir-30e-3p in inflammation mediated by the gimap8 gene. These insights contribute to our understanding of the complex mechanisms governing inflammatory and immune responses.
最近的研究越来越多地将 miRNAs 与炎症反应的调节和免疫抑制活性联系起来。这项研究表明，mir-30e-3p 选择性地结合并调节 gimap8，这一点通过荧光素酶报告基因检测和 qPCR 分析得到了证实。在 CIK 细胞受到 LPS 刺激后，mir-30e-3p 的表达显著升高，与 gimap8 mRNA 水平的下降呈负相关。过表达 mir-30e-3p 可以减弱 LPS 单独作用后的促炎细胞因子的 mRNA 水平，表明 mir-30e-3p 在 gimap8 基因介导的炎症中具有调节作用。这些发现有助于我们理解调节炎症和免疫反应的复杂机制。
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