Wei Xinfeng, Wei Wei, Liu Hongmei, Yi Junxuan, Wang Mingwei, Xu Weiqiang, Zhao Mingqi, Zhao Mengdie, Wang Rong, Jin Shunzi
NHC Key Laboratory of Radiobiology, School of Public Health, Jilin University, Changchun, Jilin, China.
Department of Radiation Oncology, Senior Department of Oncology, The Fifth Medical Center of PLA General Hospital, Beijing, China.
Sci Rep. 2025 May 2;15(1):15465. doi: 10.1038/s41598-025-99894-w.
GTPase IMAP family member 8 (GIMAP8) plays a key role in pathophysiology of several malignancies. The objective of this current research endeavor was to investigate the prognosis value of GIMAP8 in lung adenocarcinoma and examine how it relates to immunity. Expression profiles associated with GIMAP8 and related clinical details were acquired from The Cancer Genome Atlas database, and we conducted survival analysis, enrichment analysis and immune infiltration studies. Additionally, we evaluated the effect of GIMAP8 on radiation resistance of tumor by in vivo and in vitro experiments. Our results showed that lung adenocarcinoma tumor tissues exhibited lower GIMAP8 levels compared to nearby normal tissues. Furthermore, decreased GIMAP8 expression strongly correlated with poorer OS. The expression of GIMAP8 is closely related to the formation of radiation resistance in tumor cells. GSEA identified multiple signaling pathways linked to GIMAP8, including immune-related, chemokine, cell adhesion molecule, and NF-κB signaling pathways. GIMAP8 expression strongly correlated with the expression of immune checkpoint molecules, tumor mutational burden, tumor neoantigen burden, immune cells, and tumor immune microenvironment. GIMAP8 was found to have an inhibitory effect on lung adenocarcinoma and was closely related to the immune response. Moreover, GIMAP8 may also influence radiation resistance in tumors.
GTP酶IMAP家族成员8（GIMAP8）在多种恶性肿瘤的病理生理学中起关键作用。本研究的目的是探讨GIMAP8在肺腺癌中的预后价值，并研究其与免疫的关系。从癌症基因组图谱数据库中获取与GIMAP8相关的表达谱和相关临床细节，我们进行了生存分析、富集分析和免疫浸润研究。此外，我们通过体内和体外实验评估了GIMAP8对肿瘤放射抗性的影响。我们的结果表明，与附近正常组织相比，肺腺癌肿瘤组织中GIMAP8水平较低。此外，GIMAP8表达降低与较差的总生存期密切相关。GIMAP8的表达与肿瘤细胞中放射抗性的形成密切相关。基因集富集分析（GSEA）确定了多个与GIMAP8相关的信号通路，包括免疫相关、趋化因子、细胞粘附分子和NF-κB信号通路。GIMAP8表达与免疫检查点分子的表达、肿瘤突变负担、肿瘤新抗原负担、免疫细胞和肿瘤免疫微环境密切相关。发现GIMAP8对肺腺癌有抑制作用，并与免疫反应密切相关。此外，GIMAP8也可能影响肿瘤的放射抗性。
