Ouyang Yujuan, Lou Yanbing, Zhu Yanqiu, Wang Yihan, Zhu Song, Jing Lin, Yang Tingting, Cui Hengmin, Deng Huidan, Zuo Zhicai, Fang Jing, Guo Hongrui
College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China.
Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Sichuan Agriculture University, Wenjiang, Chengdu, 611130, China.
Biol Trace Elem Res. 2025 Jan;203(1):249-260. doi: 10.1007/s12011-024-04153-0. Epub 2024 Mar 26.
Selenium nanoparticle (Nano-Se) is a new type of selenium supplement, which can improve the deficiency of traditional selenium supplements and maintain its physiological activity. Due to industrial pollution and irrational use in agriculture, Cu overexposure often occurs in animals and humans. In this study, Nano-Se alleviated CuSO-induced testicular Cu accumulation, serum testosterone level decrease, testicular structural damage, and decrease in sperm quality. Meanwhile, Nano-Se reduced the ROS content in mice testis and enhanced the activities of T-AOC, GSH, SOD, and CAT compared with CuSO group. Furthermore, Nano-Se alleviated CuSO-induced apoptosis by increasing the protein expression of Cleaved-Caspase-3, Cleaved-Caspase-9, Cleaved-Caspase-12, and Bax/Bcl-2 compared with CuSO group. At the same time, Nano-Se reversed CuSO-induced increase of γ-H2AX protein expression in mice testis. In conclusion, this study confirmed that Nano-Se could alleviate oxidative stress, apoptosis, and DNA damage in the testis of mice with Cu excess, thereby protecting the spermatogenesis disorder induced by Cu.
纳米硒（Nano-Se）是一种新型的硒补充剂，它可以改善传统硒补充剂的不足并保持其生理活性。由于工业污染和农业中的不合理使用，动物和人类中经常发生铜过量暴露。在本研究中，纳米硒减轻了硫酸铜诱导的睾丸铜蓄积、血清睾酮水平降低、睾丸结构损伤以及精子质量下降。同时，与硫酸铜组相比，纳米硒降低了小鼠睾丸中的活性氧含量，并增强了总抗氧化能力（T-AOC）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。此外，与硫酸铜组相比，纳米硒通过增加裂解的半胱天冬酶-3（Cleaved-Caspase-3）、裂解的半胱天冬酶-9（Cleaved-Caspase-9）、裂解的半胱天冬酶-12（Cleaved-Caspase-12）以及Bax/Bcl-2的蛋白表达来减轻硫酸铜诱导的细胞凋亡。同时，纳米硒逆转了硫酸铜诱导的小鼠睾丸中γ-H2AX蛋白表达的增加。总之，本研究证实纳米硒可以减轻铜过量小鼠睾丸中的氧化应激、细胞凋亡和DNA损伤，从而保护由铜诱导的生精障碍。
