Guangzhou Key Laboratory of Analytical Chemistry for Biomedicine, School of Chemistry, South China Normal University, Guangzhou, Guangdong, China.
Appl Spectrosc. 2024 Jun;78(6):633-643. doi: 10.1177/00037028241238246. Epub 2024 Mar 26.
The precise regulation of nanoenzyme activity is of great significance for application to biosensing analysis. Herein, the peroxidase-like activity of carbon dots was effectively modulated by doping phosphorus, which was successfully employed for sensitive, selective detection of acid phosphatase (ACP). Phosphorus-doped carbon dots (P-CDs) with excellent peroxidase-like activity were synthesized by a one-pot hydrothermal method, and the catalytic activity could be easily modulated by controlling the additional amount of precursor phytic acid. P-CDs could effectively catalyze the oxidation of colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue TMB oxidation products in the presence of hydrogen peroxide. While ACP was able to catalyze the hydrolysis of L-ascorbyl-2-phosphate trisodium salt (AAP) to produce ascorbic acid (AA), which inhibited the peroxidase-like activity of P-CDs, by combining P-CDs nanoenzymes and ACP-catalyzed hydrolysis the colorimetric method was established for ACP detection. The absorbance variation showed a good linear relationship with ACP concentration in the range of 0.4-4.0 mU/mL with a limit of detection at 0.12 mU/mL. In addition, the method was successfully applied to detect ACP in human serum samples with recoveries in the range of 98.7-101.6%. The work provides an effective strategy for regulating nanoenzymes activity and a low-cost detection technique for ACP.
纳米酶活性的精确调控对于应用于生物传感分析具有重要意义。在此，通过掺杂磷有效地调节了碳点的过氧化物酶样活性，并成功地应用于酸性磷酸酶（ACP）的灵敏、选择性检测。通过一锅水热法合成了具有优异过氧化物酶样活性的磷掺杂碳点（P-CDs），并且可以通过控制前体植酸的添加量来轻松调节催化活性。在存在过氧化氢的情况下，P-CDs 可以有效地催化无色 3,3',5,5'-四甲基联苯胺（TMB）的氧化，生成蓝色 TMB 氧化产物。而 ACP 能够催化 L-抗坏血酸-2-磷酸三钠盐（AAP）的水解，生成抗坏血酸（AA），从而抑制 P-CDs 的过氧化物酶样活性，通过结合 P-CDs 纳米酶和 ACP 催化的水解反应，建立了用于 ACP 检测的比色法。吸光度变化与 0.4-4.0 mU/mL 范围内的 ACP 浓度呈良好的线性关系，检测限为 0.12 mU/mL。此外，该方法成功地应用于人血清样品中 ACP 的检测，回收率在 98.7-101.6%范围内。该工作为调节纳米酶活性提供了一种有效策略，并为 ACP 的低成本检测技术提供了新的思路。
