Hebei Technology Innovation Center for Energy Conversion Materials and Devices, College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang, 050024, P. R. China.
Experimental Center for Teaching, Hebei Medical University, Shijiazhuang, 050017, P. R. China.
Mikrochim Acta. 2024 Jun 11;191(7):384. doi: 10.1007/s00604-024-06456-4.
Multifunctional N, Fe-doped carbon dots (N, Fe-CDs) were synthesized by the one-step hydrothermal method using ferric ammonium citrate and dicyandiamide as raw materials. The N, Fe-CDs exhibited peroxidase-like (POD) activity by catalyzing the oxidization of 3,3',5,5'-tetramethylbenzidine (TMB) to the green oxidation state ox-TMB in the presence of hydrogen peroxide (HO). Subsequently, based on the POD activity of N, Fe-CDs, an efficient and sensitive colorimetric method for the detection of HO and ascorbic acid (AA) was established with a limit of detection of 0.40 µM and 2.05 µM. The proposed detection method has been successfully applied to detect AA in fruit juice, vitamin C tablets, and human serum samples and has exhibited excellent application prospects in biotechnology and food fields. Furthermore, N, Fe-CDs also showed a protective effect on the cell damage caused by HO and could be used as an antioxidant agent.
采用一步水热法，以柠檬酸铁铵和双氰胺为原料合成了多功能 N、Fe 掺杂碳点（N、Fe-CDs）。N、Fe-CDs 在过氧化氢（HO）存在下，通过催化 3,3',5,5'-四甲基联苯胺（TMB）氧化为绿色氧化态氧-TMB，表现出过氧化物酶样（POD）活性。随后，基于 N、Fe-CDs 的 POD 活性，建立了一种用于检测 HO 和抗坏血酸（AA）的高效灵敏比色法，检测限分别为 0.40 µM 和 2.05 µM。该检测方法已成功应用于果汁、维生素 C 片剂和人血清样本中 AA 的检测，在生物技术和食品领域具有广阔的应用前景。此外，N、Fe-CDs 还对 HO 引起的细胞损伤具有保护作用，可作为抗氧化剂使用。
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