Yu Luli, Hu Xinyao, Zhu Hua
Department of Neurosurgery, Shangrao People's Hospital, Shangrao, 334000, Jiangxi Province, China.
Cancer Center, Renmin Hospital of Wuhan University, Wuhan, 430060, China.
Heliyon. 2024 Mar 17;10(6):e28089. doi: 10.1016/j.heliyon.2024.e28089. eCollection 2024 Mar 30.
Procollagen c-protease enhancer protein (PCOLCE) performs an essential action in improving the recreation of procollagen c-protease and promoting the reconstruction of extracellular matrix. High PCOLCE expression was associated with a negative prognosis of stomach cancer, ovarian cancer, and osteosarcoma. The goal of this work is to investigate the function of PCOLCE in glioma. Multiple bioinformatics techniques have been employed to investigate the roles of PCOLCE in glioma, consisting of the correlation between PCOLCE and prognosis, immune checkpoints, immune cell infiltrates, and tumor microenvironment (TME). The gene ontology (GO) annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to assess the potential function of PCOLCE in glioma. PCOLCE was found to be increased in glioma. We revealed that PCOLCE was a potential prognostic factor and related to tumor grade. Up-regulated PCOLCE was related to poor prognosis in lower-grade glioma (LGG), glioblastoma multiforme (GBM), and recurrent glioma. PCOLCE was correlated with immune cell infiltration, particularly B cells, CD4 T cells, macrophages, neutrophils, and dendritic cells (DCs) in LGG, and DCs infiltration in GBM. PCOLCE was co-expressed with many genes related to the immune and the immune checkpoint. In addition, glioma patients with low expression of PCOLCE had a higher response to the immunological checkpoint blockade (ICB). Additionally, PCOLCE may exert its roles via several immune-related biological processes or pathways, such as leukocyte migration, activation of T cells, adaptive immune response, neutrophil-mediated immunity, NF-κB, and TNF signaling pathways. In conclusion, PCOLCE may be a new immune-related gene and regulate tumor development through immunological pathways.
前胶原C蛋白酶增强蛋白（PCOLCE）在促进前胶原C蛋白酶的再活化及推动细胞外基质重建方面发挥着至关重要的作用。PCOLCE高表达与胃癌、卵巢癌及骨肉瘤的不良预后相关。本研究旨在探究PCOLCE在胶质瘤中的功能。运用了多种生物信息学技术来研究PCOLCE在胶质瘤中的作用，包括PCOLCE与预后、免疫检查点、免疫细胞浸润及肿瘤微环境（TME）之间的相关性。采用基因本体论（GO）注释和京都基因与基因组百科全书（KEGG）分析来评估PCOLCE在胶质瘤中的潜在功能。研究发现PCOLCE在胶质瘤中表达升高。我们揭示了PCOLCE是一个潜在的预后因素且与肿瘤分级相关。PCOLCE上调与低级别胶质瘤（LGG）、多形性胶质母细胞瘤（GBM）及复发性胶质瘤的不良预后相关。PCOLCE与免疫细胞浸润相关，尤其是在LGG中与B细胞、CD4 T细胞、巨噬细胞、中性粒细胞及树突状细胞（DCs）相关，在GBM中与DCs浸润相关。PCOLCE与许多免疫及免疫检查点相关基因共表达。此外，PCOLCE低表达的胶质瘤患者对免疫检查点阻断（ICB）反应更高。另外，PCOLCE可能通过多种免疫相关生物学过程或途径发挥作用，如白细胞迁移、T细胞活化、适应性免疫反应、中性粒细胞介导的免疫、NF-κB和TNF信号通路。总之，PCOLCE可能是一个新的免疫相关基因，并通过免疫途径调节肿瘤发展。
