Fırat Kerim, Nisari Mehtap, Metin İrem, Fırat Yağmur Yaşar, Karatoprak Gökçe Şeker, Hamurcu Zühal
Department of Anatomy, Medical Faculty, Erciyes University, Kayseri 38039, Turkey.
Genome and Stem Cell Center (GENKOK), Erciyes University, Kayseri 38039, Turkey.
Toxicol Res (Camb). 2024 Mar 24;13(2):tfae046. doi: 10.1093/toxres/tfae046. eCollection 2024 Apr.
Breast cancer is one of the most common types of cancer causing high mortality rates among women worldwide. This study was aimed to evaluate the effect of (terebinth) resin extract (TRE) on the MDA-MB-231 breast cancer cell line. In the study, the cytotoxic dose of the resin extract in MDA-MB-231 cells was evaluated by MTS analysis. The effect of TRE on apoptosis was examined by Hoechst staining. Caspase-3 and cleaved caspase-3 protein expressions were determined by western blot analysis. Based on the outcomes of our MTS analysis, the IC50 dose of TRE was calculated at 56.54 μg/mL during a 24-h application period. With Hoechst staining analysis, an increase was observed in cells that underwent apoptotic change at 10 and 100 μg/ml TRE concentrations compared to the control. At 25 and 50 μg/mL TRE concentrations, no apoptotic change was found in comparison to the control; however, a significant drop in the number of viable cells was observed because 200, 300, and 500 μg/mL TRE concentrations were above the toxic dose. The caspase-3 protein expression level was significantly higher in cells treated with 100 μg/ml TRE compared to the control group, while there was no significant change in cleaved caspase-3 protein expression. It was thought that resin might cause cell death in MDA-MB-231 cells via caspase-independent apoptosis pathway or other cell death pathways, and it was concluded that it could be a supportive treatment for breast cancer treatment.
乳腺癌是全球女性中导致高死亡率的最常见癌症类型之一。本研究旨在评估松脂提取物（TRE）对MDA-MB-231乳腺癌细胞系的作用。在该研究中，通过MTS分析评估了树脂提取物在MDA-MB-231细胞中的细胞毒性剂量。通过Hoechst染色检测了TRE对细胞凋亡的影响。通过蛋白质印迹分析确定了半胱天冬酶-3和裂解的半胱天冬酶-3蛋白表达。根据我们的MTS分析结果，在24小时的作用期间，TRE的IC50剂量计算为56.54μg/mL。通过Hoechst染色分析，与对照组相比，在10和100μg/ml TRE浓度下发生凋亡变化的细胞有所增加。在25和50μg/mL TRE浓度下，与对照组相比未发现凋亡变化；然而，由于200、300和500μg/mL TRE浓度高于毒性剂量，观察到活细胞数量显著下降。与对照组相比，用100μg/ml TRE处理的细胞中半胱天冬酶-3蛋白表达水平显著更高，而裂解的半胱天冬酶-3蛋白表达没有显著变化。据认为，松脂可能通过不依赖半胱天冬酶的凋亡途径或其他细胞死亡途径导致MDA-MB-231细胞死亡，并且得出结论，它可能是乳腺癌治疗的一种辅助治疗方法。
