Putnam Christopher D
Department of Medicine, University of California, San Diego, San Diego, California, United States.
MicroPubl Biol. 2024 Mar 11;2024. doi: 10.17912/micropub.biology.001117. eCollection 2024.
DNA content measurement by fluorescence-assisted cell sorting (FACS) provides information on cell cycle progression and DNA content variability. mutants with DNA content variability that was reduced relative to wild-type strains had defects in mitochondrial DNA (mtDNA) maintenance and mitochondrial gene expression and were correlated with strains found to lack mtDNA ([ ] cells) by genome sequencing and fluorescence microscopy. In contrast, mutants with increased variability had defects in cell cycle progression, which may indicate a loss of coordination between mtDNA and nuclear DNA replication. Thus, FACS measurement of DNA content variability can provide insight into cell-to-cell heterogeneity in mtDNA copy number.
通过荧光辅助细胞分选（FACS）测量DNA含量可提供有关细胞周期进程和DNA含量变异性的信息。与野生型菌株相比，DNA含量变异性降低的突变体在线粒体DNA（mtDNA）维持和线粒体基因表达方面存在缺陷，并且与通过基因组测序和荧光显微镜检查发现缺乏mtDNA的菌株（[ ]细胞）相关。相比之下，变异性增加的突变体在细胞周期进程方面存在缺陷，这可能表明mtDNA与核DNA复制之间失去了协调性。因此，对DNA含量变异性进行FACS测量可以深入了解mtDNA拷贝数的细胞间异质性。
