Department of Electrical Engineering, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
Department of Life Sciences, Pohang University of Science and Technology (POSTECH), Pohang 37673, Republic of Korea.
Biosensors (Basel). 2024 Feb 26;14(3):124. doi: 10.3390/bios14030124.
The sensing responses of SARS-CoV-2 spike protein using top-down-fabricated Si-based electrolyte-gated transistors (EGTs) have been investigated. An aptamer was employed as a receptor for the SARS-CoV-2 spike protein. The EGT demonstrated excellent intrinsic characteristics and higher sensitivity in the subthreshold regime compared to the linear regime. The limit of detection (LOD) was achieved as low as 0.94 pg/mL and 20 pg/mL for the current and voltage sensitivity, respectively. To analyze the sensing responses of EGT in detecting the aptamer-SARS-CoV-2 spike protein conjugate, a lumped-capacitive model with the presence of an effective dipole potential and an effective capacitance of the functionalized layer component was employed. The aptamer-functionalized EGT showed high sensitivity even in 10 mM phosphate-buffered saline (PBS) solution. These results suggest that Si-based EGTs are a highly promising method for detecting SARS-CoV-2 spike proteins.
使用自上而下制造的基于硅的电解质门控晶体管（EGT）研究了 SARS-CoV-2 刺突蛋白的传感响应。适体被用作 SARS-CoV-2 刺突蛋白的受体。与线性区相比，EGT 在亚阈值区表现出优异的固有特性和更高的灵敏度。电流和电压灵敏度的检测限（LOD）分别低至 0.94 pg/mL 和 20 pg/mL。为了分析 EGT 在检测适体-SARS-CoV-2 刺突蛋白缀合物时的传感响应，采用了具有有效偶极势和功能化层组件有效电容的集总电容模型。即使在 10 mM 磷酸盐缓冲盐水（PBS）溶液中，适体功能化的 EGT 也表现出很高的灵敏度。这些结果表明，基于硅的 EGT 是一种很有前途的检测 SARS-CoV-2 刺突蛋白的方法。
