Yamaguchi Yohei, Yoshii Daiki, Katsuragi Hiroaki, Shinkai Koichi
Advance Operative Dentistry-Endodontics, The Nippon Dental University Graduate School of Life Dentistry at Niigata, 1-8 Hamauracho, Chuo-ku, Niigata 951-8580, Japan.
Department of Operative Dentistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamauracho, Chuo-ku, Niigata 951-8580, Japan.
Dent J (Basel). 2024 Mar 1;12(3):59. doi: 10.3390/dj12030059.
This study aimed to assess the impact of different laser irradiation modes and photosensitizer types on the bactericidal efficacy of antimicrobial photodynamic therapy (aPDT). Dentin plates were prepared by sectioning the crown dentin of bovine teeth infected with (n = 11). Nine aPDTs involving the combination of three 1% solutions of photosensitizers (brilliant blue, BB; acid red, AR; and methylene blue, MB) and three irradiation modes of semiconductor lasers (50 mW for 120 s, 100 mW for 60 s, and 200 mW for 30 s) were performed for each infected dentin plate, and the control consisted of the specimens not applied with aPDT. The bactericidal effects in 10 groups were evaluated using both assays of the colony count (colony-forming-unit: CFU) and adenosine triphosphate (ATP) (relative-light-unit: RLU). The data obtained were analyzed using the Kruskal-Wallis test (α = 0.05). The most aPDT groups exhibited significantly lower RLU and CFU values compared with the control ( < 0.05). The effect of irradiation modes on RLU and CFU values was significant in the aPDT group using BB ( < 0.05) but not in the aPDT group using AR or MB. The aPDT performed with AR or MB exerted a remarkable bactericidal effect.
本研究旨在评估不同激光照射模式和光敏剂类型对抗菌光动力疗法（aPDT）杀菌效果的影响。通过切割感染了[具体细菌名称未给出]（n = 11）的牛牙冠部牙本质制备牙本质片。对每块感染的牙本质片进行9种aPDT处理，这些处理涉及三种1%光敏剂溶液（亮蓝，BB；酸性红，AR；亚甲蓝，MB）与半导体激光的三种照射模式（50 mW照射120 s、100 mW照射60 s和200 mW照射30 s）的组合，对照组为未进行aPDT处理的标本。使用菌落计数（菌落形成单位：CFU）和三磷酸腺苷（ATP）（相对光单位：RLU）两种检测方法评估10组的杀菌效果。对获得的数据采用Kruskal-Wallis检验进行分析（α = 0.05）。与对照组相比，大多数aPDT组的RLU和CFU值显著更低（P < 0.05）。在使用BB的aPDT组中，照射模式对RLU和CFU值的影响显著（P < 0.05），但在使用AR或MB的aPDT组中不显著。用AR或MB进行的aPDT具有显著的杀菌效果。
