Bactericidal effect of antimicrobial photodynamic therapy (aPDT) on dentin plate infected with Lactobacillus acidophilus.

This study aimed to examine bactericidal effects of a new antimicrobial photodynamic therapy (aPDT) on dentin plates infected with Lactobacillus acidophilus (L. acidophilus). First, we measured the amount of reactive oxygen species (ROS) produced when new photosensitizer (PS), acid red (AR), and brilliant blue (BB) were irradiated with a semiconductor laser. ROS generated from each PS solution by laser irradiation was calculated as the total light emission amount (Relative Light Unit, RLU) using a chemiluminescence measuring device. Second, we examined bactericidal effects of the aPDT on dentin plates infected with L. acidophilus. The bactericidal effects on each group were evaluated by colony count assay and adenosine triphosphate assay. The experimental groups comprised two laser irradiation groups (650 nm laser, 650laser; and 940 nm laser, 940laser), two PS groups (BB and AR), four aPDT groups (650 nm laser irradiation with BB, 650laser-BB; 650 nm laser irradiation with AR, 650laser-AR; 940 nm laser irradiation with BB, 940laser-BB; 940 nm laser irradiation with AR, 940laser-AR), and a control. The ROS in all aPDT groups was significantly higher than in the control. RLU in all groups applied with laser irradiation was significantly lower than that in the control. However, only 650laser-BB showed significantly lower colony counts than the control. 650laser-BB was the most effective in sterilizing the infected dentin plates.