Annaji Manjusha, Mita Nur, Poudel Ishwor, Boddu Sai H S, Fasina Oladiran, Babu R Jayachandra
Department of Drug Discovery and Development, Harrison College of Pharmacy, Auburn University, Auburn, AL 36849, USA.
Faculty of Pharmacy, Mulawarman University, Samarinda, Kalimantan Timur 75119, Indonesia.
Bioengineering (Basel). 2024 Mar 6;11(3):259. doi: 10.3390/bioengineering11030259.
Despite rapid progress in tissue engineering, the repair and regeneration of bone defects remains challenging, especially for non-homogenous and complicated defects. We have developed and characterized biodegradable drug-eluting scaffolds for bone regeneration utilizing direct powder extrusion-based three-dimensional (3D) printing techniques. The PLGA scaffolds were fabricated using poly (lactic-co-glycolic acid) (PLGA) with inherent viscosities of 0.2 dl/g and 0.4 dl/g and ketoprofen. The effect of parameters such as the infill, geometry, and wall thickness of the drug carrier on the release kinetics of ketoprofen was studied. The release studies revealed that infill density significantly impacts the release performance, where 10% infill showed faster and almost complete release of the drug, whereas 50% infill demonstrated a sustained release. The Korsmeyer-Peppas model showed the best fit for release data irrespective of the PLGA molecular weight and infill density. It was demonstrated that printing parameters such as infill density, scaffold wall thickness, and geometry played an important role in controlling the release and, therefore, in designing customized drug-eluting scaffolds for bone regeneration.
尽管组织工程取得了快速进展，但骨缺损的修复和再生仍然具有挑战性，尤其是对于非均匀和复杂的缺损。我们利用基于直接粉末挤出的三维（3D）打印技术，开发并表征了用于骨再生的可生物降解药物洗脱支架。聚乳酸-羟基乙酸共聚物（PLGA）支架是使用特性粘度分别为0.2 dl/g和0.4 dl/g的聚（乳酸-乙醇酸）（PLGA）以及酮洛芬制备的。研究了药物载体的填充率、几何形状和壁厚等参数对酮洛芬释放动力学的影响。释放研究表明，填充密度对释放性能有显著影响，其中10%的填充率显示药物释放更快且几乎完全释放，而50%的填充率则呈现出缓释效果。无论PLGA分子量和填充密度如何，Korsmeyer-Peppas模型对释放数据的拟合效果最佳。结果表明，填充密度、支架壁厚和几何形状等打印参数在控制释放方面起着重要作用，因此在设计用于骨再生的定制药物洗脱支架中也起着重要作用。
