Nguyen Sy Le Thanh, Nguyen Thi Hien Trang, Do Thi Tuyen, Nguyen Thi Thao, Le Thanh Hoang, Nguyen Thi Anh Tuyet, Kimata Yukio
Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Road, Caugiay District, Hanoi 10600, Vietnam.
Graduate School of Science and Technology, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan.
Curr Issues Mol Biol. 2024 Feb 26;46(3):1768-1776. doi: 10.3390/cimb46030116.
Prodigiosin, a red pigment produced by numerous bacterial species, exerts various antibiotic effects on prokaryotic and eukaryotic organisms. For instance, human carcinoma cell lines appear to suffer from endoplasmic reticulum (ER) stress in the presence of prodigiosin. Here, we demonstrated that prodigiosin also triggers the unfolded-protein response (UPR), which is a cytoprotective response against ER stress, in yeast . An mutant carrying a UPR-deficient mutation was hypersensitive to prodigiosin. Our observations cumulatively indicate that protein folding in the ER is impaired by prodigiosin, illustrating a new mode of action.
灵菌红素是由多种细菌产生的一种红色色素，对原核生物和真核生物具有多种抗生素作用。例如，在灵菌红素存在的情况下，人类癌细胞系似乎会遭受内质网（ER）应激。在此，我们证明灵菌红素在酵母中也会触发未折叠蛋白反应（UPR），这是一种针对内质网应激的细胞保护反应。携带UPR缺陷突变的突变体对灵菌红素高度敏感。我们的观察结果累积表明，灵菌红素会损害内质网中的蛋白质折叠，这说明了一种新的作用模式。
