Bajic Andrej, Andersson Brittmarie, Ossinger Alexander, Tavakoli Shima, Varghese Oommen P, Schizas Nikos
Department of Surgical Sciences, Section of Orthopedics, The OrthoLab, Uppsala University, 75185 Uppsala, Sweden.
Translational Chemical Biology Laboratory, Division of Macromolecular Chemistry, Department of Chemistry-Ångstrom Laboratory, Uppsala University, 75237 Uppsala, Sweden.
Biomimetics (Basel). 2024 Feb 25;9(3):140. doi: 10.3390/biomimetics9030140.
Our aim was to investigate axonal outgrowth from different tissue models on soft biomaterials based on hyaluronic acid (HA). We hypothesized that HA-based hydrogels differentially promote axonal outgrowth from different neural tissues. Spinal cord sliced cultures (SCSCs) and dorsal root ganglion cultures (DRGCs) were maintained on a collagen gel, a physically crosslinked HA-based hydrogel (Healon 5) and a novel chemically crosslinked HA-based hydrogel, with or without the presence of neurotrophic factors (NF). Time-lapse microscopy was performed after two, five and eight days, where axonal outgrowth was assessed by automated image analysis. Neuroprotection was investigated by PCR. Outgrowth was observed in all groups; however, in the collagen group, it was scarce. At the middle timepoint, outgrowth from SCSCs was superior in both HA-based groups compared to collagen, regardless of the presence of NF. In DRGCs, the outgrowth in Healon 5 with NF was significantly higher compared to the rest of the groups. PCR revealed upregulation of NeuN gene expression in the HA-based groups compared to controls after excitotoxic injury. The differences in neurite outgrowth from the two different tissue models suggest that axons differentially respond to the two types of biomaterials.
我们的目的是研究基于透明质酸（HA）的软质生物材料上不同组织模型的轴突生长情况。我们假设基于HA的水凝胶对不同神经组织的轴突生长有不同的促进作用。将脊髓切片培养物（SCSCs）和背根神经节培养物（DRGCs）分别置于胶原凝胶、物理交联的基于HA的水凝胶（Healon 5）和新型化学交联的基于HA的水凝胶上培养，同时添加或不添加神经营养因子（NF）。在培养2天、5天和8天后进行延时显微镜观察，通过自动图像分析评估轴突生长情况。通过聚合酶链反应（PCR）研究神经保护作用。所有组均观察到轴突生长；然而，在胶原组中，轴突生长较少。在中间时间点，无论是否存在NF，基于HA的两组中SCSCs的轴突生长均优于胶原组。在DRGCs中，添加NF的Healon 5组的轴突生长明显高于其他组。PCR结果显示，与兴奋性毒性损伤后的对照组相比，基于HA的两组中NeuN基因表达上调。两种不同组织模型的神经突生长差异表明，轴突对两种生物材料的反应不同。
