Sun Fengjiang, Lv Beibei, Zhang Xuemeng, Wang Chenyu, Zhang Liyuan, Chen Xiaochen, Liang Yuancun, Chen Lei, Zou Shenshen, Dong Hansong
Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China.
Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China.
Life (Basel). 2022 May 27;12(6):799. doi: 10.3390/life12060799.
is a plant filamentous pathogenic fungi and the predominant causal agent of head blight (FHB) in cereals worldwide. The regulators of the secretory pathway contribute significantly to fungal mycotoxin synthesis, development, and virulence. However, their roles in these processes in remain poorly understood. Here, we identified and functionally characterized the endoplasmic reticulum (ER) cargo receptor FgErv14 in . Firstly, it was observed that FgErv14 is mainly localized in the ER. Then, we constructed the FgErv14 deletion mutant (Δ) and found that the absence of the FgErv14 caused a serious reduction in vegetative growth, significant defects in asexual and sexual reproduction, and severely impaired virulence. Furthermore, we found that the Δ mutant exhibited a reduced expression of genes and defective toxisome generation, both of which are critical for deoxynivalenol (DON) biosynthesis. Importantly, we found the green fluorescent protein (GFP)-tagged FgRud3 was dispersed in the cytoplasm, whereas GFP-FgSnc1-PEM was partially trapped in the late Golgi in Δ mutant. These results demonstrate that FgErv14 mediates anterograde ER-to-Golgi transport as well as late secretory Golgi-to-Plasma membrane transport and is necessary for DON biosynthesis, asexual and sexual reproduction, vegetative growth, and pathogenicity in .
是一种丝状植物病原真菌，是全球谷物中赤霉病（FHB）的主要致病因子。分泌途径的调节因子对真菌霉菌毒素的合成、发育和毒力有重要贡献。然而，它们在这些过程中的作用在[具体物种名称未给出]中仍知之甚少。在这里，我们鉴定并对[具体物种名称未给出]中的内质网（ER）货物受体FgErv14进行了功能表征。首先，观察到FgErv14主要定位于内质网。然后，我们构建了FgErv14缺失突变体（Δ），发现缺失FgErv14导致营养生长严重减少、无性和有性生殖出现显著缺陷以及毒力严重受损。此外，我们发现Δ突变体中[相关基因名称未给出]基因的表达降低且毒素体生成存在缺陷，这两者对脱氧雪腐镰刀菌烯醇（DON）的生物合成都至关重要。重要的是，我们发现绿色荧光蛋白（GFP）标记的FgRud3分散在细胞质中，而GFP - FgSnc1 - PEM在Δ突变体中部分被困在晚期高尔基体中。这些结果表明，FgErv14介导从内质网到高尔基体的顺向运输以及高尔基体到质膜的晚期分泌运输，并且是[具体物种名称未给出]中DON生物合成、无性和有性生殖、营养生长及致病性所必需的。
