Immich Felipe, de Oliveira Durvalino, Ribeiro de Andrade Juliana Silva, da Silva Barboza Andressa, Cuevas-Suárez Carlos Enrique, da Silva Adriana Fernandes, de Oliveira da Rosa Wellington Luiz, Borges Álvaro Henrique, Carreno Neftali Lenin Villarreal, Piva Evandro, Guerra Lund Rafael
Graduate Program in Dentistry, School of Dentistry, Federal University of Pelotas (UFPEL), Pelotas 96015-560, Brazil.
Graduate Program in Dentistry, School of Dentistry, University of Cuiabá (UNIC), Cuiabá 78000-000, Brazil.
J Funct Biomater. 2024 Mar 14;15(3):70. doi: 10.3390/jfb15030070.
This study aimed to evaluate the antimicrobial properties, cell viability, and matrix metalloproteinase (MMP) inhibition capacity of several endodontic materials aimed at vital pulp therapy: Pro Root MTA, EndoSequence, Biodentine, MTA Angelus, TheraCal LC, and BioC Repair. The materials were prepared according to the manufacturer's instructions. Antimicrobial tests were conducted using a microcosm biofilm model, cell viability was assessed using murine fibroblasts (L929), and MMP activity was analyzed through electrophoresis. The results showed that BioC Repair, Biodentine, and EndoSequence exhibited similar antimicrobial properties, while MTA Angelus and ProRoot MTA had inferior results but were comparable to each other. In terms of cell viability, no significant differences were observed among the materials. EndoSequence demonstrated the highest MMP inhibition capacity. In conclusion, BioC Repair, Biodentine, EndoSequence, and TheraCal showed better antimicrobial properties among the tested materials. The materials did not exhibit significant differences in terms of cytotoxicity. However, EndoSequence displayed superior MMP inhibition capacity.
本研究旨在评估几种用于活髓治疗的根管治疗材料的抗菌性能、细胞活力和基质金属蛋白酶（MMP）抑制能力：Pro Root MTA、EndoSequence、Biodentine、MTA Angelus、TheraCal LC和BioC Repair。材料按照制造商的说明制备。使用微观生物膜模型进行抗菌测试，使用小鼠成纤维细胞（L929）评估细胞活力，并通过电泳分析MMP活性。结果表明，BioC Repair、Biodentine和EndoSequence表现出相似的抗菌性能，而MTA Angelus和ProRoot MTA的结果较差，但彼此相当。在细胞活力方面，各材料之间未观察到显著差异。EndoSequence表现出最高的MMP抑制能力。总之，在测试材料中，BioC Repair、Biodentine、EndoSequence和TheraCal表现出更好的抗菌性能。这些材料在细胞毒性方面没有显著差异。然而，EndoSequence表现出卓越的MMP抑制能力。
