细菌纤维素与纳米壳聚糖点的光活性复合材料在蓝光和绿光下对致病性生物膜的减少作用

Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light.

作者信息

Zmejkoski Danica Z, Zdravković Nemanja M, Budimir Filimonović Milica D, Pavlović Vladimir B, Butulija Svetlana V, Milivojević Dušan D, Marković Zoran M, Todorović Marković Biljana M

机构信息

Vinča Institute of Nuclear Sciences, National Institute of the Republic of Serbia, University of Belgrade, 11001 Belgrade, Serbia.

Scientific Institute for Veterinary Medicine of Serbia, Janisa Janulisa 14, 11107 Belgrade, Serbia.

出版信息

J Funct Biomater. 2024 Mar 14;15(3):72. doi: 10.3390/jfb15030072.

In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5-2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections.

在本研究中，利用伽马射线合成了纳米壳聚糖点（ChiDs），并将其封装在细菌纤维素（BC）聚合物基质中，用于光动力疗法中的抗生物膜潜力研究。使用扫描电子显微镜（SEM）、原子力显微镜（AFM）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、电子顺磁共振（EPR）和孔隙率测量等方法对复合材料的结构变化进行了分析。此外，还评估了ChiD的释放情况。结果表明，化学成分保持不变，但嵌入BC中的ChiD团聚体形状发生了变化（1.5 - 2.5 µm）。细菌纤维素纤维发生变形并相互连接，表面粗糙度和孔隙率增加，结晶度降低。未观察到单线态氧的形成，ChiD的总释放量高达16.10%。绿光下抗生物膜活性更高，蓝光下降低幅度为48%至57%，绿光下为78%至85%。耐甲氧西林金黄色葡萄球菌是最敏感的菌株。新型光活性复合水凝胶在对抗生物膜相关感染方面显示出有前景的潜力。