School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China.
School of Materials Science and Engineering, University of Science and Technology Beijing, Beijing, 100083, PR China.
Mater Sci Eng C Mater Biol Appl. 2020 Jan;106:110249. doi: 10.1016/j.msec.2019.110249. Epub 2019 Oct 14.
Biofilm-associated infections are in a high rate of recurrence and biofilms show formidable resistance to current antibiotics, making them a growing challenge in biomedical field. In this study, a biocompatible composite was developed by incorporating tannic acid (TA) and MgCl to bacterial cellulose (BC) for antimicrobial and anti-biofilm purposes. The morphology was investigated by scanning electron microscopy (SEM), and chemical structure were characterized by Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectra (XPS). In vitro release profiles of tannic acid revealed that the Mg cross-links help impede the release of TA from BC matrix, while composite BC-TA lacked Mg ionic cross-links, thus more TA was released from the hydrogel. The BC-TA-Mg composites also displayed strong antibacterial activity against S. aureus, E. coli and P. aeruginosa. Moreover, the composites significantly reduced biofilm formation of S. aureus and P. aeruginosa after 24 h incubation by ∼80% and ∼87%, respectively. As a consequence, the BC-TA-Mg composites are a very promising material for combating biofilm-associated infections in biomedical and public health fields.
生物膜相关感染的复发率很高，生物膜对目前的抗生素表现出很强的耐药性，这使得它们成为生物医学领域的一个日益严峻的挑战。在这项研究中，通过将单宁酸（TA）和 MgCl 掺入细菌纤维素（BC）中，开发了一种具有生物相容性的复合材料，用于抗菌和抗生物膜目的。通过扫描电子显微镜（SEM）研究了形态，通过傅里叶变换红外光谱（FTIR）和 X 射线光电子能谱（XPS）对化学结构进行了表征。单宁酸的体外释放曲线表明，Mg 交联有助于阻止 TA 从 BC 基质中释放，而缺乏 Mg 离子交联的复合 BC-TA 则从水凝胶中释放出更多的 TA。BC-TA-Mg 复合材料还对金黄色葡萄球菌、大肠杆菌和铜绿假单胞菌表现出强烈的抗菌活性。此外，复合材料在 24 小时孵育后分别显著降低了金黄色葡萄球菌和铜绿假单胞菌的生物膜形成，降低幅度分别约为 80%和 87%。因此，BC-TA-Mg 复合材料是对抗生物医学和公共卫生领域生物膜相关感染的一种很有前途的材料。
