Carvalho Augusto M, Costa Rúbia S, Lago Alexsandro, Bacellar Olívia, Beiting Daniel P, Scott Phillip, Carvalho Lucas P, Carvalho Edgar M
Gonçalo Moniz Institute (IGM), Fiocruz, Salvador 40296-710, BA, Brazil.
Immunology Service, Professor Edgard Santos University Hospital Complex, Federal University of Bahia, Salvador 40110-160, BA, Brazil.
Pathogens. 2024 Feb 23;13(3):199. doi: 10.3390/pathogens13030199.
The role of the immune response in the pathogenesis of cutaneous leishmaniasis (CL) due to is predominantly carried out via blood cells. Here, we evaluate whether cytokine production by peripheral blood mononuclear cells (PBMCs) reflects what has been documented at the lesion site. The participants included 22 CL patients diagnosed with a positive PCR. PBMCs were stimulated for 72 h with a soluble leishmania antigen (SLA). Biopsies obtained from the edge of the ulcers were incubated for the same period. Cytokines in supernatants were assessed via ELISA. TNF, IL-1β, IL-6, IL-17, and granzyme B (GzmB) were higher in the supernatants of biopsies than in PBMCs, but IFN-γ was higher in the supernatants of PBMCs than in biopsies. There was a positive correlation between IFN-γ and TNF in PBMCs, and an inverse correlation between TNF and IL-10 in the cells from the lesion site. A strong correlation between IL-1β, IL-17, and GzmB was observed in the biopsies, and a positive correlation was detected between these cytokines and the lesion size. Our results indicate that the immune response in lesions is different from that observed in peripheral blood, and our data suggest that in addition to IL-1β and GzmB, IL-17 participates in the pathology of CL.
免疫反应在由[病原体名称未给出]引起的皮肤利什曼病（CL）发病机制中的作用主要通过血细胞来实现。在此，我们评估外周血单个核细胞（PBMCs）产生的细胞因子是否反映了病变部位已记录的情况。研究参与者包括22名经PCR检测呈阳性的CL患者。用可溶性利什曼原虫抗原（SLA）刺激PBMCs 72小时。从溃疡边缘获取的活检组织在相同时间段内进行培养。通过酶联免疫吸附测定法（ELISA）评估上清液中的细胞因子。活检组织上清液中的肿瘤坏死因子（TNF）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）、白细胞介素-17（IL-17）和颗粒酶B（GzmB）高于PBMCs中的水平，但PBMCs上清液中的干扰素-γ（IFN-γ）高于活检组织中的水平。PBMCs中IFN-γ与TNF呈正相关，病变部位细胞中TNF与白细胞介素-1（IL-10）呈负相关。在活检组织中观察到IL-1β、IL-17和GzmB之间有很强的相关性，并且在这些细胞因子与病变大小之间检测到正相关。我们的结果表明，[病原体名称未给出]病变中的免疫反应与外周血中观察到的不同，并且我们的数据表明，除了IL-1β和GzmB之外，IL-17也参与CL的病理过程。
