Senyagin Alexandr, Sachivkina Nadezhda, Das Milana, Arsenyuk Anna, Mannapova Ramziya, Mannapov Alfir, Kubatbekov Tursumbai, Svistunov Dmitriy, Petrukhina Olesya, Zharov Andrey, Zhabo Natallia
Department of Microbiology V.S. Kiktenko, Medical Institute, RUDN University Named after Patrice Lumumba, 117198 Moscow, Russia.
All-Russian Research Institute for Veterinary Sanitation, Hygiene and Ecology-Branch of Federal Scientific Center-K.I. Skryabin, Ya.R. Kovalenko All-Russian Research Institute of Experimental Veterinary Medicine, Russian Academy of Sciences, 109428 Moscow, Russia.
Pathogens. 2024 Mar 15;13(3):252. doi: 10.3390/pathogens13030252.
Urinary tract infections occupy a special niche among diseases of infectious etiology. Many microorganisms associated with urinary tract infections, such as , spp., , , , , , spp., and spp., can form biofilms. The aim of this research was to study the effect of the enzyme L-lysine-Alpha-oxidase (LO) produced by the fungus on the biofilm formation process of microorganisms associated with urinary tract infections. Homogeneous LO showed a more pronounced effect than the culture liquid concentrate (cCL). When adding samples at the beginning of incubation, the maximum inhibition was observed in relation to 5960-cCL 86%, LO 95%; 1669-cCL 85%, LO 94%; 6392-cCL 83%, LO-98%; and 3057-cCL 70%, LO-82%. The minimum inhibition was found in spp. Scanning electron microscopy was carried out, and numerous morphological and structural changes were observed in the cells after culturing the bacterial cultures in a medium supplemented with homogeneous LO. For example, abnormal division was detected, manifesting as the appearance of joints in places where the bacteria diverge. Based on the results of this work, we can draw conclusions about the possibility of inhibiting microbial biofilm formation with the use of LO; especially significant inhibition was achieved when the enzyme was added at the beginning of incubation. Thus, LO can be a promising drug candidate for the treatment or prevention of infections associated with biofilm formation.
尿路感染在感染性病因疾病中占据特殊地位。许多与尿路感染相关的微生物，如 、 属、 、 、 、 、 属和 属，都能形成生物膜。本研究的目的是研究真菌产生的L - 赖氨酸 - α - 氧化酶（LO）对与尿路感染相关微生物生物膜形成过程的影响。纯LO比培养液浓缩物（cCL）显示出更显著的效果。在培养开始时添加样品时，观察到对 5960 - cCL抑制率为86%，LO为95%；对 1669 - cCL抑制率为85%，LO为94%；对 6392 - cCL抑制率为83%，LO为98%；对 3057 - cCL抑制率为70%，LO为82%。在 属中发现的抑制作用最小。进行了扫描电子显微镜观察，在补充了纯LO的培养基中培养细菌培养物后，观察到细胞出现了许多形态和结构变化。例如，检测到异常分裂，表现为细菌分叉处出现连接处。基于这项工作的结果，我们可以得出关于使用LO抑制微生物生物膜形成可能性的结论；当在培养开始时添加该酶时，实现了特别显著的抑制。因此，LO可能是治疗或预防与生物膜形成相关感染的有前景的候选药物。
