NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Woodbridge Rd, Menangle NSW 2568, Australia.
University of Technology Sydney, 15 Broadway, Ultimo NSW 2007, Australia.
Int J Syst Evol Microbiol. 2024 Mar;74(3). doi: 10.1099/ijsem.0.006294.
Five bacterial isolates were isolated from in 1976 in Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these isolates represented members of the genus . To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic analysis involved using MALDI-TOF MS and BIOLOG GEN III microplates, which confirmed that the isolates represented members of the genus but did not allow them to be classified with respect to species. Genome relatedness indices and the results of extensive phylogenetic analysis confirmed that the isolates were members of the genus and represented a novel species. On the basis the minimal presence of virulence-associated factors typically found in genomes of members of the genus , we suggest that these isolates are non-pathogenic. This conclusion was supported by the results of a pathogenicity assay. On the basis of these findings, we propose the name , with DAR 34855 = ICMP 24941 as the type strain.
1976 年，在澳大利亚雷德尔默尔（Rydalmere）进行常规生物安全监测时，从 中分离出了 5 株细菌分离株。最初，生化特性分析的结果表明，这些分离株代表了 属的成员。为了确定它们的种，进一步使用表型和基因型方法进行了分析。表型分析涉及使用 MALDI-TOF MS 和 BIOLOG GEN III 微板，这证实了分离株代表了 属的成员，但无法对其进行种的分类。基因组相关指数和广泛的系统发育分析结果证实，这些分离株是 属的成员，代表了一个新的种。基于通常在 属成员基因组中发现的毒力相关因子的最低存在，我们认为这些分离株是无致病性的。致病性测定的结果支持了这一结论。基于这些发现，我们提出了 的名称，其中 DAR 34855 = ICMP 24941 作为模式株。
