Department of Pediatrics, Chi Mei Medical Center, Tainan, 710, Taiwan.
Department of Biotechnology and Food Technology, Southern Taiwan University of Science and Technology, Tainan, 710, Taiwan.
Sci Rep. 2024 Mar 27;14(1):7244. doi: 10.1038/s41598-024-57706-7.
We aimed to evaluate whether white and gray matter microstructure changes observed with magnetic resonance imaging (MRI)-based diffusion tensor imaging (DTI) can be used to reflect the progression of chronic brain trauma. The MRI-DTI parameters, neuropathologic changes, and behavioral performance of adult male Wistar rats that underwent moderate (2.1 atm on day "0") or repeated mild (1.5 atm on days "0" and "2") traumatic brain injury (TBI or rmTBI) or sham operation were evaluated at 7 days, 14 days, and 1-9 months after surgery. Neurobehavioral tests showed that TBI causes long-term motor, cognitive and neurological deficits, whereas rmTBI results in more significant deficits in these paradigms. Both histology and MRI show that rmTBI causes more significant changes in brain lesion volumes than TBI. In vivo DTI further reveals that TBI and rmTBI cause persistent microstructural changes in white matter tracts (such as the body of the corpus callosum, splenium of corpus callus, internal capsule and/or angular bundle) of both two hemispheres. Luxol fast blue measurements reveal similar myelin loss (as well as reduction in white matter thickness) in ipsilateral and contralateral hemispheres as observed by DTI analysis in injured rats. These data indicate that the disintegration of microstructural changes in white and gray matter parameters analyzed by MRI-DTI can serve as noninvasive and reliable markers of structural and functional level alterations in chronic TBI.
我们旨在评估磁共振成像（MRI）-弥散张量成像（DTI）观察到的白质和灰质微观结构变化是否可用于反映慢性脑创伤的进展。评估了成年雄性 Wistar 大鼠的 MRI-DTI 参数、神经病理学变化和行为表现，这些大鼠接受了中度（第“0”天 2.1 大气压）或重复轻度（第“0”和“2”天 1.5 大气压）创伤性脑损伤（TBI 或 rmTBI）或假手术，分别在手术后 7 天、14 天和 1-9 个月进行。神经行为学测试表明，TBI 导致长期的运动、认知和神经功能缺陷，而 rmTBI 在这些范式中导致更显著的缺陷。组织学和 MRI 均显示，rmTBI 引起的脑损伤体积变化比 TBI 更显著。体内 DTI 进一步显示，TBI 和 rmTBI 导致两个半球的白质束（如胼胝体体部、胼胝体压部、内囊和/或角束）的微观结构变化持续存在。卢索快速蓝测量显示，在损伤大鼠的 DTI 分析中观察到同侧和对侧半球的髓鞘丢失（以及白质厚度减少）相似。这些数据表明，MRI-DTI 分析的白质和灰质参数的微观结构变化的解体可以作为慢性 TBI 结构和功能水平改变的无创和可靠标志物。
