Science and Biomedical Doctoral Programme, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia.
Department of Parasitology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia.
Malar J. 2024 Mar 27;23(1):88. doi: 10.1186/s12936-024-04888-0.
Anopheles vagus (subgenus Cellia) has been identified as a vector for malaria, filariasis, and Japanese encephalitis in Asia. Sporozoites of Plasmodium falciparum and Plasmodium vivax have been found in this zoophilic mosquito in Asia and Indonesia. This study systematically reviews publications regarding An. vagus species, variation, bio-ecology, and malaria transmission in various localities in Asia, especially Indonesia, to determine whether the current data support An. vagus as a species complex.
The databases Pubmed, Scopus, Europe PMC, and Proquest were searched to identify information regarding the morphology, karyotypes, polytene chromosome, cross-mating, ecology, and molecular identification of An. vagus was then evaluated to determine whether there were possible species complexes.
Of the 1326 articles identified, 15 studies were considered for synthesis. The Anopheles spp. samples for this study came from Asia. Eleven studies used morphology to identify An. vagus, with singular studies using each of karyotype identification, chromosomal polytene identification, and cross-breeding experiments. Ten studies used molecular techniques to identify Anopheles spp., including An. vagus. Most studies discovered morphological variations of An. vagus either in the same or different areas and ecological settings. In this review, the members of An. vagus sensu lato grouped based on morphology (An. vagus, An. vagus vagus, An. vagus limosus, and An. limosus), karyotyping (form A and B), and molecular (An. vagus genotype A and B, An. vagus AN4 and AN5). Genetic analysis revealed a high conservation of the ITS2 fragment among members except for the An. vagus genotype B, which was, in fact, Anopheles sundaicus. This review also identified that An. vagus limosus and An. vagus vagus were nearly identical to the ITS2 sequence.
Literature review studies revealed that An. vagus is conspecific despite the distinct morphological characteristic of An. vagus and An. limosus. Further information using another barcoding tool, such as mitochondrial COI and ND6 and experimental cross-mating between the An. vagus and An. limosus may provide additional evidence for the status of An. vagus as a species complex.
致倦库蚊（属 Cellia 亚属）已被确定为亚洲疟疾、丝虫病和日本脑炎的传播媒介。在亚洲和印度尼西亚，这种嗜人按蚊中发现了恶性疟原虫和间日疟原虫的孢子。本研究系统地综述了有关致倦库蚊种、变异、生物生态以及亚洲不同地区（特别是印度尼西亚）疟疾传播的文献，以确定当前数据是否支持致倦库蚊为一个物种复合体。
检索了 Pubmed、Scopus、Europe PMC 和 Proquest 数据库，以确定有关致倦库蚊形态、核型、多线染色体、杂交、生态学和分子鉴定的信息，然后评估是否存在可能的物种复合体。
在确定的 1326 篇文章中，有 15 项研究被认为可用于综合分析。本研究的按蚊样本来自亚洲。11 项研究使用形态学来鉴定致倦库蚊，而单独的研究使用核型鉴定、染色体多线染色体鉴定和杂交实验。10 项研究使用分子技术鉴定按蚊属，包括致倦库蚊。大多数研究在同一地区或不同地区和生态环境中发现致倦库蚊的形态变异。在本综述中，根据形态学（致倦库蚊、致倦库蚊 vagus、致倦库蚊 limosus 和致倦库蚊 limosus）、核型（A 型和 B 型）和分子（致倦库蚊基因型 A 和 B、致倦库蚊 AN4 和 AN5）对致倦库蚊 sensu lato 的成员进行分组。遗传分析表明，除致倦库蚊基因型 B 外，ITS2 片段在成员之间高度保守，而致倦库蚊基因型 B 实际上是印度尼西亚按蚊。本综述还发现，致倦库蚊 limosus 和致倦库蚊 vagus 与 ITS2 序列几乎相同。
文献综述研究表明，尽管致倦库蚊和致倦库蚊 limosus 具有明显的形态特征，但致倦库蚊是同一种。使用另一种条形码工具（如线粒体 COI 和 ND6 以及致倦库蚊和致倦库蚊 limosus 之间的实验杂交）获得更多信息，可能为致倦库蚊作为一个物种复合体的地位提供额外证据。
