Andrade Daniela, Maldonado-Bravo Francisca, Alburquerque Amador, Pérez Camilo, Gamboa Alexander, Caro Nelson, Díaz-Dosque Mario, Gotelland Martin, Abugoch Lilian, Tapia Cristian
Department of Food Science and Chemical Technology, Faculty of Chemical Sciences and Pharmacy, University of Chile, Carlos Lorca Tobar 964, Independencia, Santiago 8380494, Chile.
Department of Environmental Sciences, Faculty of Chemistry and Biology, University of Santiago de Chile, Santiago 9170022, Chile.
Antioxidants (Basel). 2024 Feb 23;13(3):273. doi: 10.3390/antiox13030273.
Maqui berries contain a high percentage of anthocyanins with high antioxidant and anti-inflammatory capacity but that are unstable in the colonic site. Nanocarriers based on polysaccharides and/or proteins can protect against the degradation of anthocyanins. The aim of this study was the nanoencapsulation of maqui extract (ME) in chitosan-tripolyphosphate (CTPP-ME), chenopodin (CH-ME), and chenopodin-alginate (CHA-ME). A standardised ME was prepared and then encapsulated in the nanosystems. The physicochemical properties, encapsulation parameters, and the interactions of ME with the nanovehicles were characterised. The cyanidin-3-glucoside released and ORAC activity in phosphate buffer at pH 7.4 were evaluated. The content of ME was 8-9 mg of cyanidin-3-glucoside/g of extract. CTPP with ME at 3% obtained the highest encapsulation efficiency (EE = 91%), and no significant differences were observed in size (274-362 nm), PDI (0.5-0.7), and zeta potential (+34-+41 mV) when the concentration of ME changed from 1% to 5%. CH-ME was shown to be smaller (152 nm) than CTPP-ME, and CH-ME and CHA-ME showed lower EE (79% and 54%, respectively) than CTPP-ME. FT-IR revealed a stronger interaction of ME with CTPP-ME than with CH-ME. Both systems showed a significantly lower release than free ME, and the T50 value of CTPP-ME 3% (328 min) was higher than CH-ME (197 min). Both protected the ORAC activity of ME.
马基莓含有高比例的花青素，具有高抗氧化和抗炎能力，但在结肠部位不稳定。基于多糖和/或蛋白质的纳米载体可以防止花青素降解。本研究的目的是将马基提取物（ME）纳米包封在壳聚糖-三聚磷酸钠（CTPP-ME）、藜科植物蛋白（CH-ME）和藜科植物蛋白-海藻酸盐（CHA-ME）中。制备了标准化的ME，然后将其包封在纳米系统中。对其物理化学性质、包封参数以及ME与纳米载体的相互作用进行了表征。评估了pH 7.4的磷酸盐缓冲液中矢车菊素-3-葡萄糖苷的释放和氧自由基吸收能力（ORAC）活性。ME的含量为8-9毫克矢车菊素-3-葡萄糖苷/克提取物。含3%ME的CTPP获得了最高的包封效率（EE = 91%），当ME浓度从1%变化到5%时，在粒径（274-362纳米）、多分散指数（PDI，0.5-0.7）和zeta电位（+34-+41毫伏）方面未观察到显著差异。CH-ME的粒径显示比CTPP-ME小（152纳米），并且CH-ME和CHA-ME的EE低于CTPP-ME（分别为79%和54%）。傅里叶变换红外光谱（FT-IR）显示ME与CTPP-ME的相互作用比与CH-ME的更强。两种体系释放的药物均显著低于游离ME，3%CTPP-ME的T50值（328分钟）高于CH-ME（197分钟）。两者都保护了ME的ORAC活性。
