Lee Hana, Wang Zhixin, Deng Zhanao, Wang Yu
Department of Food Science and Human Nutrition, Citrus Research and Education Center, University of Florida, Lake Alfred, FL 33850, USA.
Department of Environmental Horticulture, Gulf Coast Research and Education Center, University of Florida, Institute of Food and Agricultural Sciences, Wimauma, FL 33598, USA.
Antioxidants (Basel). 2024 Mar 6;13(3):319. doi: 10.3390/antiox13030319.
Blackberries have gained considerable attention due to their high antioxidant content and potential health benefits. This study compared the metabolite profiles of six blackberry cultivars and investigated their biological activities. The metabolites extracted from blackberries were analyzed using metabolomics, and their biological activities and mechanisms were confirmed using in vitro models and network pharmacology. Among the cultivars examined, "Kiowa" ripe berries exhibited the highest antioxidant and anti-inflammatory activities. These effects were primarily attributed to the accumulation of flavonoids (quercitrin and luteolin) and anthocyanin (cyanidin 3--glucoside) in the phenylpropanoid pathway. Furthermore, our research identified 13 blackberry metabolites that interacted with 31 genes, including AKT1, CASP3, JUN, MAPK8, NOS3, NQO1, and HMOX1 which play roles in reducing oxidative stress, protecting cells from damage, and suppressing inflammation. These findings suggest that blackberry metabolites, such as quercitrin, luteolin, and cyanidin 3--glucoside, may exert therapeutic effects by modulating specific genes and pathways associated with antioxidant and anti-inflammatory responses. This research is promising not only for plant breeders but also for those interested in harnessing the health-promoting properties of blackberries.
黑莓因其高抗氧化剂含量和潜在的健康益处而备受关注。本研究比较了六个黑莓品种的代谢物谱，并研究了它们的生物活性。使用代谢组学分析从黑莓中提取的代谢物，并使用体外模型和网络药理学确认其生物活性和作用机制。在所检测的品种中，“基奥瓦”成熟果实表现出最高的抗氧化和抗炎活性。这些作用主要归因于苯丙烷途径中黄酮类化合物（槲皮苷和木犀草素）和花青素（矢车菊素3 - 葡萄糖苷）的积累。此外，我们的研究鉴定出13种黑莓代谢物与31个基因相互作用，包括AKT1、CASP3、JUN、MAPK8、NOS3、NQO1和HMOX1，这些基因在减轻氧化应激、保护细胞免受损伤和抑制炎症方面发挥作用。这些发现表明，黑莓代谢物，如槲皮苷、木犀草素和矢车菊素3 - 葡萄糖苷，可能通过调节与抗氧化和抗炎反应相关的特定基因和途径发挥治疗作用。这项研究不仅对植物育种者有希望，而且对那些对利用黑莓促进健康特性感兴趣的人也有希望。
