Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Jiangsu Key Laboratory for the Research and Utilization of Plant Resources, The Jiangsu Provincial Platform for Conservation and Utilization of Agricultural Germplasm, Qian Hu Hou Cun No. 1, Nanjing 210014, China.
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, 159 Longpan Road, Nanjing 210037, China.
Food Res Int. 2022 Mar;153:110948. doi: 10.1016/j.foodres.2022.110948. Epub 2022 Jan 12.
Blackberry fruit is rich in anthocyanins, showing incomparable nutritional and health value. Anthocyanins are flavonoids that are downstream products of the flavonoid biosynthesis pathway. They are important secondary metabolites with antioxidant and anticancer functions. Therefore, we performed combined transcriptome and metabolome analyses of unripe and ripe blackberry fruits to identify the molecular mechanisms of flavonoid biosynthesis. Herein, we identified 997 differentially expressed metabolites (DEMs) in positive-ion mode, 411 DEMs in negative-ion mode, and 17,566 differentially expressed genes (DEGs). Eight DEMs and 16 DEGs were annotated to the flavonoid biosynthesis pathway. Four DEGs related to flavonoids (unigene 0028088, unigene 0039337, unigene 0063209, and unigene 0014842) were positively correlated with related flavonoid DEMs. Interestingly, 8 DEGs and 11 flavonoid-related DEMs showed significant negative correlations. This study lays a foundation for further research on the biosynthesis and metabolism of flavonoid, providing a reference for the future breeding and cultivation of excellent high-flavonoid germplasm resources and the blackberry-related pharmaceutical industry.
黑莓果实富含花色苷，具有无与伦比的营养价值和健康价值。花色苷是类黄酮的下游产物，属于黄酮类生物合成途径的产物。它们是具有抗氧化和抗癌功能的重要次生代谢物。因此，我们对未成熟和成熟的黑莓果实进行了联合转录组和代谢组分析，以鉴定类黄酮生物合成的分子机制。在此，我们在正离子模式下鉴定了 997 个差异表达代谢物（DEMs），在负离子模式下鉴定了 411 个 DEMs 和 17566 个差异表达基因（DEGs）。8 个 DEM 和 16 个 DEG 被注释到类黄酮生物合成途径。4 个与类黄酮相关的 DEG（unigene0028088、unigene0039337、unigene0063209 和 unigene0014842）与相关类黄酮 DEM 呈正相关。有趣的是，8 个 DEG 和 11 个类黄酮相关的 DEM 表现出显著的负相关。本研究为进一步研究类黄酮的生物合成和代谢奠定了基础，为未来优异高类黄酮种质资源的选育和栽培以及黑莓相关制药行业提供了参考。
