Yu Heng, Masagounder Karthik, Liang Hualiang, Ge Xianping, Huang Dongyu, Xue Chunyu, Ren Mingchun, He Juyun
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
Evonik Operations GmbH, Hanau-Wolfgang, 63457 Hanau, Germany.
Antioxidants (Basel). 2024 Mar 18;13(3):359. doi: 10.3390/antiox13030359.
DL-methionyl-DL-methionine (AQUAVI® Met-Met) (Met-Met) (0.10%, 0.20%, 0.30%, and 0.40%) or DL-methionine (DL-Met) (0.10%, 0.20%, 0.30%, and 0.40%) were added to a low-fishmeal diet in an attempt to reduce fishmeal in the diet of (). The fish were randomly allocated into ten experimental groups ( = 100), each with 4 replicates of 25 fish (16.39 ± 0.01 g) each. Compared to 25% FM, 0.40% of DL-Met and 0.10% of Met-Met promoted growth, and 0.10% of Met-Met decreased FCR. Compared to 25% FM, the supplementation of Met-Met or DL-Met improved the intestinal antioxidant capacity by upregulating the NF-E2-related factor 2-mediated antioxidant factors and enzyme activities and nuclear factor kappa-B-mediated anti-inflammatory factors while downregulating the pro-inflammatory factors, thereby exerting anti-inflammatory effects. Moreover, 0.10% of the Met-Met diet affected the Firmicutes-to-Bacteroidota ratio, increased the levels of Proteobacteria, changed the composition of intestinal flora (, , and ), and enhanced intestinal dominant bacteria (, , and ), leading to improved gut health. In summary, the supplementation of DL-Met or Met-Met alleviated the adverse effect of fishmeal reduction (from 40 to 25%) on the growth performance and intestinal health of .
将DL-蛋氨酰-DL-蛋氨酸（AQUAVI® Met-Met）（蛋氨酸-蛋氨酸）（0.10%、0.20%、0.30%和0.40%）或DL-蛋氨酸（DL-Met）（0.10%、0.20%、0.30%和0.40%）添加到低鱼粉日粮中，以尝试减少（）日粮中的鱼粉含量。将鱼随机分为十个实验组（每组n = 100），每组有4个重复，每个重复25尾鱼（体重16.39±0.01克）。与25%鱼粉组相比，0.40%的DL-蛋氨酸和0.10%的蛋氨酸-蛋氨酸促进生长，0.10%的蛋氨酸-蛋氨酸降低饲料系数。与25%鱼粉组相比，补充蛋氨酸-蛋氨酸或DL-蛋氨酸可通过上调NF-E2相关因子2介导的抗氧化因子和酶活性以及核因子κB介导的抗炎因子，同时下调促炎因子来提高肠道抗氧化能力，从而发挥抗炎作用。此外，0.10%蛋氨酸-蛋氨酸日粮影响厚壁菌门与拟杆菌门的比例，增加变形菌门水平，改变肠道菌群组成（、和），并增强肠道优势菌（、和），从而改善肠道健康。总之，补充DL-蛋氨酸或蛋氨酸-蛋氨酸可减轻鱼粉减少（从40%降至25%）对（）生长性能和肠道健康的不利影响。
