Berry Danyale, Ene Justice, Nathani Aakash, Singh Mandip, Li Yan, Zeng Changchun
Department of Industrial and Manufacturing Engineering, FAMU-FSU College of Engineering, Florida Agricultural and Mechanical University, Tallahassee, FL 32310, USA.
High Performance Materials Institute, FAMU-FSU College of Engineering, Florida State University, Tallahassee, FL 23210, USA.
Biomedicines. 2024 Feb 22;12(3):489. doi: 10.3390/biomedicines12030489.
The peripheral nervous system undergoes sufficient stress when affected by diabetic conditions, chemotherapeutic drugs, and personal injury. Consequently, peripheral neuropathy arises as the most common complication, leading to debilitating symptoms that significantly alter the quality and way of life. The resulting chronic pain requires a treatment approach that does not simply mask the accompanying symptoms but provides the necessary external environment and neurotrophic factors that will effectively facilitate nerve regeneration. Under normal conditions, the peripheral nervous system self-regenerates very slowly. The rate of progression is further hindered by the development of fibrosis and scar tissue formation, which does not allow sufficient neurite outgrowth to the target site. By incorporating scaffolding supplemented with secretome derived from human mesenchymal stem cells, it is hypothesized that neurotrophic factors and cellular signaling can facilitate the optimal microenvironment for nerve reinnervation. However, conventional methods of secretory vesicle production are low yield, thus requiring improved methods to enhance paracrine secretions. This report highlights the state-of-the-art methods of neuropathy treatment as well as methods to optimize the clinical application of stem cells and derived secretory vesicles for nerve regeneration.
当受到糖尿病病情、化疗药物和人身伤害影响时，外周神经系统会承受足够的压力。因此，周围神经病变成为最常见的并发症，导致使人衰弱的症状，显著改变生活质量和生活方式。由此产生的慢性疼痛需要一种治疗方法，这种方法不仅能简单地掩盖伴随症状，还能提供必要的外部环境和神经营养因子，有效促进神经再生。在正常情况下，外周神经系统自我再生非常缓慢。纤维化和瘢痕组织形成的发展进一步阻碍了进展速度，这使得神经突无法充分生长到目标部位。通过加入补充了人骨髓间充质干细胞分泌组的支架，推测神经营养因子和细胞信号传导可以促进神经再支配的最佳微环境。然而，传统的分泌囊泡生产方法产量较低，因此需要改进方法来增强旁分泌分泌。本报告重点介绍了神经病变治疗的最新方法，以及优化干细胞及其衍生分泌囊泡在神经再生临床应用的方法。
