Department of Plant Production, Misión Biológica de Galicia (CSIC), Avda de Vigo s/n, 15705 Santiago de Compostela, Spain.
Genes (Basel). 2024 Mar 21;15(3):388. doi: 10.3390/genes15030388.
Wounding and exogenous auxin are needed to induce adventitious roots in chestnut microshoots. However, the specific inductive role of wounding has not been characterized in this species. In the present work, two main goals were established: First, we prompted to optimize exogenous auxin treatments to improve the overall health status of the shoots at the end of the rooting cycle. Second, we developed a time-series transcriptomic analysis to compare gene expression in response to wounding alone and wounding plus auxin, focusing on the early events within the first days after treatments. Results suggest that the expression of many genes involved in the rooting process is under direct or indirect control of both stimuli. However, specific levels of expression of relevant genes are only attained when both treatments are applied simultaneously, leading to the successful development of roots. In this sense, we have identified four transcription factors upregulated by auxin (, , and ), with some of them also being induced by wounding. The highest expression levels of these genes occurred when wounding and auxin treatments were applied simultaneously, correlating with the rooting response of the shoots. The results of this work clarify the genetic nature of the wounding response in chestnut, its relation to adventitious rooting, and might be helpful in the development of more specific protocols for the vegetative propagation of this species.
创伤和外源生长素是诱导板栗微芽产生不定根所必需的。然而，这种物种的创伤的具体诱导作用尚未被描述。在本研究中，确立了两个主要目标：首先，我们试图优化外源生长素处理，以提高生根周期结束时芽的整体健康状况。其次，我们进行了时间序列转录组分析，以比较单独创伤和创伤加生长素处理的基因表达情况，重点关注处理后最初几天内的早期事件。结果表明，许多参与生根过程的基因的表达受到这两种刺激的直接或间接控制。然而，只有当同时应用这两种处理时，才能达到相关基因的特定表达水平，从而成功地发育出根。在这个意义上，我们已经鉴定出了四个受生长素上调的转录因子（、、和），其中一些也受到创伤的诱导。这些基因的最高表达水平出现在创伤和生长素处理同时应用时，与芽的生根反应相关。这项工作的结果阐明了板栗创伤反应的遗传本质，它与不定根的形成的关系，并且可能有助于开发更具体的该物种营养繁殖的方案。
