Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Room 317 Biomedical Research Bldg., 3435 Main St., Buffalo, NY 14214, USA.
Division of Infectious Diseases, Department of Medicine, Jacobs School of Medicine and Biomedical Sciences, University at Buffalo, Room 317 Biomedical Research Bldg., 3435 Main St., Buffalo, NY 14214, USA.
Biomolecules. 2024 Mar 8;14(3):321. doi: 10.3390/biom14030321.
Our laboratory recently reported that induction of the SOS response, triggered by SOS-inducing drugs, was accompanied by a large release of DNA from enteric bacteria. The SOS response release had not previously been reported to include release of extracellular DNA from bacterial cells. We followed up on those observations in this current study and found that not just double-stranded DNA was being released, but also single-stranded DNA, RNA, and protein. SOS-inducing drugs also triggered formation of biofilm at the air-fluid interface on glass, and the biofilms contained DNA. We extended our study to test whether inhibitors of the SOS response would block DNA release and found that SOS inhibitors, including zinc salts, nitric oxide donors, and dequalinium, inhibited SOS-induced DNA release. The understanding that SOS-induced DNA release is associated with formation of biofilms increases our appreciation of the role of the SOS response in pathogenesis, as well as in emergence of new antibiotic resistance. Our findings with SOS inhibitors also suggest that regimens might be devised that could block the deleterious effects of the SOS response, at least temporarily, when this is desired.
我们实验室最近报告称，SOS 诱导药物引发的 SOS 反应伴随着肠道细菌大量释放 DNA。此前，SOS 反应释放并未被报道包括细菌细胞中外源 DNA 的释放。在本研究中，我们对这些观察结果进行了跟进，发现不仅释放了双链 DNA，还释放了单链 DNA、RNA 和蛋白质。SOS 诱导药物还会在玻璃的气液界面触发生物膜的形成，而生物膜中含有 DNA。我们进一步研究以测试 SOS 反应抑制剂是否会阻断 DNA 释放，发现 SOS 抑制剂，包括锌盐、一氧化氮供体和癸甲氯铵，可抑制 SOS 诱导的 DNA 释放。SOS 诱导的 DNA 释放与生物膜形成有关的认识增加了我们对 SOS 反应在发病机制以及新抗生素耐药性出现中的作用的认识。我们使用 SOS 抑制剂的研究结果还表明，在需要时，可能会设计出方案来阻止 SOS 反应的有害影响，至少是暂时的。
