• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

通过金纳米颗粒和顺铂处理对肺泡腺癌细胞中热休克蛋白表达的调节

Modulation of Heat Shock Protein Expression in Alveolar Adenocarcinoma Cells through Gold Nanoparticles and Cisplatin Treatment.

作者信息

Ibrahim Bashiru, Akere Taiwo Hassan, Chakraborty Swaroop, Valsami-Jones Eugenia, Ali-Boucetta Hanene

机构信息

Nanomedicine, Drug Delivery & Nanotoxicology (NDDN) Lab, School of Pharmacy, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, UK.

School of Geography, Earth and Environmental Sciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham B15 2TT, UK.

出版信息

Pharmaceutics. 2024 Mar 11;16(3):380. doi: 10.3390/pharmaceutics16030380.

DOI:10.3390/pharmaceutics16030380
PMID:38543274
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10974746/
Abstract

Heat-shock proteins (HSPs) are stress-responsive molecules belonging to the family of evolutionary molecular chaperones known to be crucial in many cancer types, including human alveolar adenocarcinoma cells (A549). These proteins are highly overexpressed in cancers to support their ability to accommodate imbalances in cell signalling, DNA alterations, proteins, and energy metabolism associated with oncogenesis. The current study evaluated the effects of gold nanoparticles (AuNPs) combined with cisplatin (CDDP) on molecular chaperone HSPs in A549 cells. It was found that AuNPs:CDDP decreased the percentage of cell viability (38.5%) measured using the modified lactated dehydrogenase (mLDH) and 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assays. AuNPs:CDDP exposure caused a significant ( < 0.05) increase in reactive oxygen species (ROS) generation by 1.81-fold, apoptosis induction, and a decrease in the mitochondrial membrane potential (MMP) compared to AuNPs or CDDP alone. Similarly, exposure to the AuNPs:CDDP combination had pronounced cytotoxic effects on the expression of HSPs and PI3K/AKT/mTOR, as well as apoptosis-related proteins. The results demonstrate that the combination of AuNPs with CDDP might enhance the anticancer efficacy of CDDP.

摘要

热休克蛋白（HSPs）是应激反应分子，属于进化分子伴侣家族，已知在包括人肺泡腺癌细胞（A549）在内的许多癌症类型中起关键作用。这些蛋白质在癌症中高度过表达，以支持其适应与肿瘤发生相关的细胞信号传导失衡、DNA改变、蛋白质和能量代谢的能力。本研究评估了金纳米颗粒（AuNPs）与顺铂（CDDP）联合使用对A549细胞中分子伴侣HSPs的影响。研究发现，AuNPs:CDDP降低了使用改良乳酸脱氢酶（mLDH）和3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）测定法测得的细胞活力百分比（38.5%）。与单独使用AuNPs或CDDP相比，AuNPs:CDDP暴露导致活性氧（ROS）生成显著增加（<0.05）1.81倍，诱导凋亡，并降低线粒体膜电位（MMP）。同样，暴露于AuNPs:CDDP组合对HSPs和PI3K/AKT/mTOR以及凋亡相关蛋白的表达具有明显的细胞毒性作用。结果表明，AuNPs与CDDP联合使用可能会增强CDDP的抗癌疗效。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/00deb4181b18/pharmaceutics-16-00380-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/fb8b44087fb0/pharmaceutics-16-00380-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/a84f4d408472/pharmaceutics-16-00380-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/84dfc3bdbc06/pharmaceutics-16-00380-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/d5da7c768c0b/pharmaceutics-16-00380-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/bff67f4f995f/pharmaceutics-16-00380-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/a2a34d0d8184/pharmaceutics-16-00380-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/00deb4181b18/pharmaceutics-16-00380-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/fb8b44087fb0/pharmaceutics-16-00380-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/a84f4d408472/pharmaceutics-16-00380-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/84dfc3bdbc06/pharmaceutics-16-00380-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/d5da7c768c0b/pharmaceutics-16-00380-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/bff67f4f995f/pharmaceutics-16-00380-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/a2a34d0d8184/pharmaceutics-16-00380-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/005c/10974746/00deb4181b18/pharmaceutics-16-00380-g007.jpg

相似文献

1
Modulation of Heat Shock Protein Expression in Alveolar Adenocarcinoma Cells through Gold Nanoparticles and Cisplatin Treatment.通过金纳米颗粒和顺铂处理对肺泡腺癌细胞中热休克蛋白表达的调节
Pharmaceutics. 2024 Mar 11;16(3):380. doi: 10.3390/pharmaceutics16030380.
2
Functionalized Gold Nanoparticles Suppress the Proliferation of Human Lung Alveolar Adenocarcinoma Cells by Deubiquitinating Enzymes Inhibition.功能化金纳米颗粒通过抑制去泛素化酶来抑制人肺腺癌细胞的增殖。
ACS Omega. 2023 Oct 20;8(43):40622-40638. doi: 10.1021/acsomega.3c05452. eCollection 2023 Oct 31.
3
Gold Nanoparticles Induced Size Dependent Cytotoxicity on Human Alveolar Adenocarcinoma Cells by Inhibiting the Ubiquitin Proteasome System.金纳米颗粒通过抑制泛素蛋白酶体系统对人肺泡腺癌细胞产生大小依赖性细胞毒性。
Pharmaceutics. 2023 Jan 28;15(2):432. doi: 10.3390/pharmaceutics15020432.
4
Gambogic acid synergistically potentiates cisplatin-induced apoptosis in non-small-cell lung cancer through suppressing NF-κB and MAPK/HO-1 signalling.藤黄酸通过抑制 NF-κB 和 MAPK/HO-1 信号通路协同增强顺铂诱导的非小细胞肺癌细胞凋亡。
Br J Cancer. 2014 Jan 21;110(2):341-52. doi: 10.1038/bjc.2013.752. Epub 2013 Dec 3.
5
[Wogonoside reverses cisplatin resistance in SGC7901/cDDP cells through inhibition of PI3K/Akt/Nrf2/ARE signaling pathway].[汉黄芩苷通过抑制PI3K/Akt/Nrf2/ARE信号通路逆转SGC7901/cDDP细胞对顺铂的耐药性]
Sheng Li Xue Bao. 2018 Aug 25;70(4):397-405.
6
[RNA Interference targeting GRP75 decreases cisplatin resistance in human lung adenocarcinoma cell].靶向GRP75的RNA干扰降低人肺腺癌细胞的顺铂耐药性
Zhongguo Fei Ai Za Zhi. 2011 Apr;14(4):305-10. doi: 10.3779/j.issn.1009-3419.2011.04.01.
7
Neferine and isoliensinine enhance 'intracellular uptake of cisplatin' and induce 'ROS-mediated apoptosis' in colorectal cancer cells - A comparative study.荷叶碱和莲心碱增强结直肠癌细胞“顺铂的细胞内摄取”并诱导“ROS 介导的细胞凋亡”- 一项比较研究。
Food Chem Toxicol. 2019 Oct;132:110652. doi: 10.1016/j.fct.2019.110652. Epub 2019 Jun 27.
8
[Effect of down-regulation of survivin gene on apoptosis and cisplatin resistance in cisplatin resistant human lung adenocarcinoma A549/CDDP cells].[生存素基因下调对顺铂耐药人肺腺癌A549/CDDP细胞凋亡及顺铂耐药性的影响]
Zhonghua Zhong Liu Za Zhi. 2006 Jun;28(6):408-12.
9
The aqueous extract of cinnamon bark ameliorated cisplatin-induced cytotoxicity in vero cells without compromising the anticancer efficiency of cisplatin.肉桂皮水提取物改善了顺铂诱导的 vero 细胞毒性，且不影响顺铂的抗癌效率。
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2016 Sep;160(3):363-71. doi: 10.5507/bp.2016.034. Epub 2016 Jul 25.
10
Mitochondrial DNA mutations are involved in the acquisition of cisplatin resistance in human lung cancer A549 cells.线粒体 DNA 突变参与人肺癌 A549 细胞顺铂耐药的获得。
Oncol Rep. 2022 Feb;47(2). doi: 10.3892/or.2021.8243. Epub 2021 Dec 22.

本文引用的文献

1
Functionalized Gold Nanoparticles Suppress the Proliferation of Human Lung Alveolar Adenocarcinoma Cells by Deubiquitinating Enzymes Inhibition.功能化金纳米颗粒通过抑制去泛素化酶来抑制人肺腺癌细胞的增殖。
ACS Omega. 2023 Oct 20;8(43):40622-40638. doi: 10.1021/acsomega.3c05452. eCollection 2023 Oct 31.
2
Gold nanoparticles and gold nanorods in the landscape of cancer therapy.金纳米粒子和金纳米棒在癌症治疗领域的应用。
Mol Cancer. 2023 Jun 21;22(1):98. doi: 10.1186/s12943-023-01798-8.
3
Selamectin increases cisplatin sensitivity by inhibiting cisplatin-resistant genes expression and autophagy in uveal melanoma.
塞拉菌素通过抑制葡萄膜黑色素瘤中顺铂耐药基因的表达和自噬来提高顺铂敏感性。
Biochem Biophys Res Commun. 2023 Jun 18;661:75-81. doi: 10.1016/j.bbrc.2023.04.008. Epub 2023 Apr 14.
4
Gold Nanoparticles Induced Size Dependent Cytotoxicity on Human Alveolar Adenocarcinoma Cells by Inhibiting the Ubiquitin Proteasome System.金纳米颗粒通过抑制泛素蛋白酶体系统对人肺泡腺癌细胞产生大小依赖性细胞毒性。
Pharmaceutics. 2023 Jan 28;15(2):432. doi: 10.3390/pharmaceutics15020432.
5
Roles of reactive oxygen species, mitochondrial membrane potential, and p53 in evodiamine-induced apoptosis and G2/M arrest of human anaplastic thyroid carcinoma cells.活性氧、线粒体膜电位和p53在吴茱萸碱诱导人未分化甲状腺癌细胞凋亡及G2/M期阻滞中的作用
Chin Med. 2021 Dec 9;16(1):134. doi: 10.1186/s13020-021-00505-3.
6
Synergistic effects of silver nanoparticles and cisplatin in combating inflammation and hyperplasia of airway stents.银纳米颗粒与顺铂在对抗气道支架炎症和增生方面的协同作用。
Bioact Mater. 2021 Jul 29;9:266-280. doi: 10.1016/j.bioactmat.2021.07.029. eCollection 2022 Mar.
7
The Pathogenic Role of PI3K/AKT Pathway in Cancer Onset and Drug Resistance: An Updated Review.PI3K/AKT信号通路在癌症发生和耐药中的致病作用：最新综述
Cancers (Basel). 2021 Aug 5;13(16):3949. doi: 10.3390/cancers13163949.
8
Recent advances in heat shock proteins in cancer diagnosis, prognosis, metabolism and treatment.癌症诊断、预后、代谢和治疗中的热休克蛋白的最新进展。
Biomed Pharmacother. 2021 Oct;142:112074. doi: 10.1016/j.biopha.2021.112074. Epub 2021 Aug 20.
9
Heat Shock Protein 90 Triggers Multi-Drug Resistance of Ovarian Cancer via AKT/GSK3β/β-Catenin Signaling.热休克蛋白90通过AKT/GSK3β/β-连环蛋白信号通路引发卵巢癌的多药耐药性。
Front Oncol. 2021 Mar 2;11:620907. doi: 10.3389/fonc.2021.620907. eCollection 2021.
10
Silver nanoparticle induced toxicity and cell death mechanisms in embryonic zebrafish cells.银纳米颗粒诱导斑马鱼胚胎细胞毒性和细胞死亡的机制。
Nanoscale. 2021 Mar 28;13(12):6142-6161. doi: 10.1039/d0nr09024g. Epub 2021 Mar 18.