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基于非洲猪瘟病毒和牛痘病毒序列同源性鉴定潜在的进入融合复合物。

Identification of a Potential Entry-Fusion Complex Based on Sequence Homology of African Swine Fever and Vaccinia Virus.

机构信息

Departamento de Biotecnología, INIA-CSIC, Centro Nacional Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Ctra. de la Coruña Km 7.5, 28040 Madrid, Spain.

Unidad BICS, Centro de Investigaciones Biológicas Margarita Salas, Consejo Superior de Investigaciones Científicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.

出版信息

Viruses. 2024 Feb 23;16(3):349. doi: 10.3390/v16030349.

DOI:10.3390/v16030349
PMID:38543715
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10975062/
Abstract

African swine fever virus (ASFV) belongs to the family of , part of the group of nucleocytoplasmic large DNA viruses (NCLDV). Little is known about the internalization of ASFV in the host cell and the fusion membrane events that take place at early stages of the infection. Poxviruses, also members of the NCLDV and represented by vaccinia virus (VACV), are large, enveloped, double-stranded DNA viruses. Poxviruses were considered unique in having an elaborate entry-fusion complex (EFC) composed of 11 highly conserved proteins integrated into the membrane of mature virions. Recent advances in methodological techniques have again revealed several connections between VACV EFC proteins. In this study, we explored the possibility of an analogous ASFV EFC by identifying ten candidate proteins exhibiting structural similarities with VACV EFC proteins. This could reveal key functions of these ASFV proteins, drawing attention to shared features between the two virus families, suggesting the potential existence of an ASFV entry-fusion complex.

摘要

非洲猪瘟病毒（ASFV）属于 科，是核质大 DNA 病毒（NCLDV）组的一部分。目前对于 ASFV 在宿主细胞内的内化以及感染早期发生的融合膜事件知之甚少。痘病毒也是 NCLDV 的成员，以牛痘病毒（VACV）为代表，是大型包膜双链 DNA 病毒。痘病毒被认为具有独特的精心设计的进入融合复合物（EFC），由 11 种高度保守的蛋白质整合到成熟病毒粒子的膜中。最近方法学技术的进步再次揭示了 VACV EFC 蛋白之间的几个联系。在这项研究中，我们通过鉴定十个候选蛋白，这些蛋白与 VACV EFC 蛋白具有结构相似性，探索了类似的 ASFV EFC 的可能性。这可能揭示这些 ASFV 蛋白的关键功能，引起人们对两个病毒家族之间共享特征的关注，表明 ASFV 进入融合复合物的潜在存在。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/ebb78dd15298/viruses-16-00349-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/5fa43669ef0e/viruses-16-00349-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/69fa6d864c05/viruses-16-00349-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/3199517228cd/viruses-16-00349-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/b199b30f6a00/viruses-16-00349-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/c02808c660f8/viruses-16-00349-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/49094c4461a9/viruses-16-00349-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/ebb78dd15298/viruses-16-00349-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/5fa43669ef0e/viruses-16-00349-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/69fa6d864c05/viruses-16-00349-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/3199517228cd/viruses-16-00349-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/b199b30f6a00/viruses-16-00349-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/c02808c660f8/viruses-16-00349-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/49094c4461a9/viruses-16-00349-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/37b9/10975062/ebb78dd15298/viruses-16-00349-g007.jpg

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3
Structural and functional analysis of vaccinia viral fusion complex component protein A28 through NMR and molecular dynamic simulations.
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4
Impact of AlphaFold on structure prediction of protein complexes: The CASP15-CAPRI experiment.AlphaFold 对蛋白质复合物结构预测的影响：CASP15-CAPRI 实验。
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5
Protein sequence comparison based on representation on a finite dimensional unit hypercube.基于有限维单位超正方体表示的蛋白质序列比较。
J Biomol Struct Dyn. 2024 Aug;42(12):6425-6439. doi: 10.1080/07391102.2023.2268719. Epub 2023 Oct 14.
6
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7
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