Department of Entomology, Plant Pathology, and Nematology, University of Idaho, 875 Perimeter Drive, MS 2329, Moscow, ID 83844-2329, USA.
Department of Entomology, Plant Pathology, and Nematology, and Kimberly Research and Extension Center, University of Idaho, Kimberly, ID 83341-5082, USA.
Viruses. 2024 Mar 8;16(3):415. doi: 10.3390/v16030415.
An investigation of viruses circulating in populations of field and laboratory potato/tomato psyllids () was conducted using high-throughput sequencing (HTS) technology and conventional RT-PCR. Three new viruses were discovered: one from the family and two from the family . A tymo-like virus sequence represented a nearly complete 6843 nt genome of a virus named Bactericera cockerelli tymo-like virus (BcTLV) that spanned five open reading frames (ORFs) which encoded RNA-dependent RNA polymerase (RdRP), helicase, protease, methyltransferase, and a capsid protein. Phylogenetic analyses placed the RdRP of BcTLV inside a divergent lineage of the viruses from the family found in insect and plant hosts in a sister clade to the genera , , and . Four solemo-like virus sequences were identified in the HTS outputs, representing two new viruses. One virus found only in field-collected psyllids and named Bactericera cockerelli solemo-like virus 1 (BcSLV-1) had a 5479 nt genome which spanned four ORFs encoding protease and RdRP. Three solemo-like sequences displayed 87.4-99.7% nucleotide sequence identity among themselves, representing variants or strains of the same virus named Bactericera cockerelli solemo-like virus 2 (BcSLV-2). The genome of BcSLV-2 spanned only two ORFs that encoded a protease and an RdRP. Phylogenetic analysis placed the RdRPs of BcSLV-1 and BcSLV-2 in two separate lineages as sister clades to viruses from the genus found in plant hosts. All three new psyllid viruses were found circulating in psyllids collected from potato fields in southern Idaho along with a previously identified Bactericera cockerelli picorna-like virus. Any possible role of the three viruses in controlling populations of the field psyllids remains to be elucidated.
采用高通量测序（HTS）技术和常规 RT-PCR 对田间和实验室马铃薯/番茄木虱种群中循环的病毒进行了调查。发现了三种新病毒：一种来自，两种来自。一种拟病毒样病毒序列代表了一种名为烟粉虱拟病毒（BcTLV）的病毒的近完整 6843 个核苷酸基因组，该病毒跨越五个开放阅读框（ORF），编码 RNA 依赖性 RNA 聚合酶（RdRP）、解旋酶、蛋白酶、甲基转移酶和衣壳蛋白。系统发育分析将 BcTLV 的 RdRP 置于昆虫和植物宿主中发现的病毒科内的一个分化谱系内，与属、、和处于姐妹分支。在 HTS 输出中鉴定出 4 个索莱莫样病毒序列，代表两种新病毒。一种仅在田间采集的木虱中发现的病毒命名为烟粉虱索莱莫样病毒 1（BcSLV-1），其 5479 个核苷酸基因组跨越四个 ORF，编码蛋白酶和 RdRP。三个索莱莫样序列彼此之间显示出 87.4-99.7%的核苷酸序列同一性，代表同一病毒的变体或株系，命名为烟粉虱索莱莫样病毒 2（BcSLV-2）。BcSLV-2 的基因组仅跨越两个 ORF，编码蛋白酶和 RdRP。系统发育分析将 BcSLV-1 和 BcSLV-2 的 RdRPs 置于两个单独的分支中，与在植物宿主中发现的属的病毒形成姐妹分支。在爱达荷州南部马铃薯田中采集的木虱中发现了所有三种新的粉虱病毒，以及先前鉴定的烟粉虱微小 RNA 病毒。三种病毒在控制田间木虱种群中的任何可能作用仍有待阐明。
