Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, P.R. China.
School of Basic Medical Sciences, Yunnan University of Chinese Medicine, Kunming 650500, P.R. China.
J Agric Food Chem. 2024 Apr 10;72(14):7991-8005. doi: 10.1021/acs.jafc.3c09753. Epub 2024 Mar 28.
The biotransformation of patchouli alcohol by afforded 31 products, including 21 new ones (-, , , -, and -). Their structures were determined by extensive spectroscopic data analysis (H and C NMR, HSQC, HMBC, H-H COSY, ROESY, and HRESIMS), and the absolute configuration of compounds , , , , and was determined by single-crystal X-ray diffraction using Cu Kα radiation. Structurally, compounds were patchoulol-type norsesquiterpenoids without Me-12. Among them, a Δ double bond existed in compounds and ; a three-membered ring was formed between C-4, C-5, and C-6 in compound ; an epoxy moiety appeared between C-3 and C-4 in compound . Furthermore, the biotransformation products , , and showed potent anti-influenza virus activity with EC values of 2.11, 7.94, 20.87, and 3.45 M, respectively.
通过 生物转化 提供了 31 种产物，包括 21 种新产物（-,,, -, 和 -）。它们的结构通过广泛的光谱数据分析（H 和 C NMR、HSQC、HMBC、H-H COSY、ROESY 和 HRESIMS）确定，并且通过单晶 X 射线衍射使用 Cu Kα 辐射确定了化合物 、 、 、 和 的绝对构型。在结构上，化合物 为无 Me-12 的派秋醇型倍半萜。其中，在化合物 和 中存在 Δ 双键；在化合物 中，C-4、C-5 和 C-6 之间形成了一个三元环；在化合物 中，C-3 和 C-4 之间出现了一个环氧部分。此外，生物转化产物 、 、 和 表现出很强的抗流感病毒活性，EC 值分别为 2.11、7.94、20.87 和 3.45 μM。
