Adams Latif, Obiri-Yeboah Dorcas, Afiadenyo Michael, Hamidu Sherif, Aning Abigail, Ehun Ebenezer, Shiels Katie, Joshi Akanksha, Mamfe Sakyimah Maxwell, Asamoah Kusi Kwadwo, Ayi Irene, Mckeon Bennett Michelle, Moane Siobhan
Technological University of Shannon: Midlands Midwest, Midlands Campus, Athlone, Ireland.
Department of Microbiology and Immunology, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, Ghana.
Heliyon. 2024 Mar 16;10(6):e28025. doi: 10.1016/j.heliyon.2024.e28025. eCollection 2024 Mar 30.
African Trypanosomiasis caused by trypanosome parasites continues to be a major neglected health problem, particularly in developing countries. Current treatments are marked by serious side effects, low effectiveness, high toxicity, and drug resistance prompting the need to develop novel, safe, effective, and alternative antitrypanosomal compounds. is an ethnomedicinal plant used in West Africa to treat many ailments including protozoan diseases. In this study, we investigated the antitrypanosomal potential of stem bark extracts of through and approaches. extracts were tested for their antitrypanosomal activities against parasite using Alamar blue assay. In addition, the antioxidant and cytotoxic activities were determined. LC-ESI-QTOF-MS was used to identify potential bioactive compounds present in the extracts. Bioactive compounds identified were subjected to molecular docking studies against (TR) and Uridine Diphosphate Galactose 4'-Epimerase (UDP). The . extracts (methanol, hexane, chloroform, and ethyl acetate) exhibited potential anti-trypanosomal activities with IC values of 21.25 ± 0.755,4.35 ± 0.166,2.57 ± 0.153 and 22.92 ± 2.321 μg/mL respectively. Moreover, the methanolic crude extracts showed moderate cytotoxicity against HepG2 and PNT2 cells, with IC values of 68.0 ± 2.05 and 78.7 ± 2.63 μg/mL respectively. LC-MS analysis revealed the presence of 24 bioactive compounds with 5 being druglike. Risperidone, Ranolazine, Dihydro-7-Desacetyldeoxygedunin, 6 beta-Hydroxytriamcinolone acetonide, and Dimethylmatairesinol were identified as novel potential inhibitors of TR and UDP with binding affinities of -10.4, -7.9, -8.7, -8.4 and -7.1 kcal/mol respectively against TR and -10.8, -8.4, -8.4, -7.6 and -8.1 respectively against UDP. This study indicates that has potential antitrypanosomal properties and therefore may have the potential to be developed as a therapeutic intervention for treating African trypanosomiasis.
由锥虫寄生虫引起的非洲锥虫病仍然是一个主要的被忽视的健康问题，特别是在发展中国家。目前的治疗方法存在严重的副作用、低效、高毒性和耐药性等问题，这促使人们需要开发新型、安全、有效且替代的抗锥虫化合物。[植物名称]是一种在西非用于治疗包括原生动物疾病在内的多种疾病的民族药用植物。在本研究中，我们通过[具体方法1]和[具体方法2]方法研究了[植物名称]茎皮提取物的抗锥虫潜力。使用Alamar蓝分析法测试了[植物名称]提取物对[寄生虫名称]寄生虫的抗锥虫活性。此外，还测定了其抗氧化和细胞毒性活性。采用液相色谱-电喷雾-四极杆飞行时间质谱法（LC-ESI-QTOF-MS）鉴定了[植物名称]提取物中存在的潜在生物活性化合物。对鉴定出的生物活性化合物进行了针对[酶名称1]（TR）和尿苷二磷酸半乳糖4'-表异构酶（UDP）的分子对接研究。[植物名称]提取物（甲醇、己烷、氯仿和乙酸乙酯）表现出潜在的抗锥虫活性，IC50值分别为21.25±0.755、4.35±0.166、2.57±0.153和22.92±2.321μg/mL。此外，甲醇粗提物对HepG2和PNT2细胞表现出中度细胞毒性，IC50值分别为68.0±2.05和78.7±2.63μg/mL。LC-MS分析显示存在24种生物活性化合物，其中5种具有类药物性质。利培酮、雷诺嗪、二氢-7-去乙酰脱氧姜黄酮、6β-羟基曲安奈德丙酮化物和二甲基matairesinol被鉴定为TR和UDP的新型潜在抑制剂，它们对TR的结合亲和力分别为-10.4、-7.9、-8.7、-8.4和-7.1kcal/mol，对UDP的结合亲和力分别为-10.8、-8.4、-8.4、-7.6和-8.1。本研究表明[植物名称]具有潜在的抗锥虫特性，因此可能有潜力被开发为治疗非洲锥虫病的治疗干预措施。
