Sarıçoban Barış, Kuru Murat, Fındık Sıddıka, Kılınç İbrahim, Altınok Tamer
Department of Chest Surgery, Bitlis Tatvan State Hospital, Bitlis, Türkiye.
Department of Chest Surgery, Necmettin Erbakan University Faculty of Medicine, Konya, Türkiye.
Turk Gogus Kalp Damar Cerrahisi Derg. 2024 Jan 29;32(1):62-68. doi: 10.5606/tgkdc.dergisi.2024.25516. eCollection 2024 Jan.
The aim of this study was to investigate the antifibrinolytic and anti-inflammatory effects of hesperidin, tenoxicam and enoxaparin on intrapleural adhesions in an experimental rat model.
A total of 52 healthy adult male Wistar Albino rats from the same colony were randomly divided into six groups as sham (Group 1), surgical control (Group 2), low-dose hesperidin (Group 3), high-dose hesperidin (Group 4), tenoxicam (Group 5), and enoxaparin (Group 6). All subjects underwent left thoracotomy and except for the sham group, an adhesion model was applied and, postoperatively, the drugs were administered intraperitoneally. On Day 11 postoperatively, the rats were sacrificed and their blood levels of interleukin-1β and interleukin-10 were examined and they were evaluated for pleural adhesion area, adhesion severity score, mesothelial cell proliferation score, mononuclear cell infiltration score, and macrophage infiltration score in the collagen layer.
The lowest adhesion area and adhesion severity score were found in Group 6. There was a statistically significant difference between Group 2 and Group 6 and between Group 3 and Group 6 in terms of both parameters (p=0.04 and p=0.02). As for adhesion area, a statistically significant difference was found between Group 5 and Group 6 (p=0.04). Statistically significant differences were also found between Group 2 and Group 5 in terms of mesothelial cell proliferation scores and between Group 1 and Group 4 in terms of mononuclear cell infiltration scores (p=0.03 and p=0.02).
Enoxaparin, tenoxicam, and high-dose hesperidin act at different points to prevent adhesion in rats.
本研究旨在探讨橙皮苷、替诺昔康和依诺肝素对实验性大鼠模型胸膜粘连的抗纤维蛋白溶解和抗炎作用。
将来自同一群体的52只健康成年雄性Wistar白化大鼠随机分为六组，即假手术组（第1组）、手术对照组（第2组）、低剂量橙皮苷组（第3组）、高剂量橙皮苷组（第4组）、替诺昔康组（第5组）和依诺肝素组（第6组）。所有受试动物均接受左胸切开术，除假手术组外，均应用粘连模型，术后腹腔内给药。术后第11天，处死大鼠，检测其血液中白细胞介素-1β和白细胞介素-10水平，并评估其胸膜粘连面积、粘连严重程度评分、间皮细胞增殖评分、单核细胞浸润评分和胶原层巨噬细胞浸润评分。
第6组的粘连面积和粘连严重程度评分最低。在这两个参数方面，第2组和第6组之间以及第3组和第6组之间存在统计学显著差异（p=0.04和p=0.02）。就粘连面积而言，第5组和第6组之间存在统计学显著差异（p=0.04）。在间皮细胞增殖评分方面，第2组和第5组之间也存在统计学显著差异，在单核细胞浸润评分方面，第1组和第4组之间存在统计学显著差异（p=0.03和p=0.02）。
依诺肝素、替诺昔康和高剂量橙皮苷在不同点发挥作用以预防大鼠粘连。
