Bu Guanhao, Zhao Xiaoling, Wang Mengli, Ti Guanghui, Chen Fusheng, Duan Xiaojie, Huang Yanan, Li Panxin
College of Food Science and Engineering, Henan University of Technology, Zhengzhou, China.
Henan Province Nanjie Village (Group) Co., Ltd, Luohe, China.
J Sci Food Agric. 2024 Aug 30;104(11):6676-6686. doi: 10.1002/jsfa.13493. Epub 2024 Apr 12.
Peanut peptides have good chelating ability with metal ions. However, there are few studies on the chelation mechanism of peanut peptides with calcium and absorption properties of peptide-calcium complex.
Peptides with high calcium chelating rate were isolated and purified from peanut protein hydrolysate (PPH), and the chelation rate of component F21 was higher (81.4 ± 0.8%). Six peptides were identified from component F21 by liquid chromatography-tandem mass spectrometry, and the frequency of acidic amino acids and arginine in the amino acid sequence was higher in all six peptides. Peanut peptide-calcium complex (PPH21-Ca) was prepared by selecting component F21 (PPH21). Ultraviolet analysis indicated that the chelate reaction occurred between peanut peptide and calcium ions. Fourier transform infrared analysis showed that the chelating sites were carboxyl and amino groups on the amino acid residues of peptides. Scanning electron microscopy revealed that the surface of peanut peptide had a smooth block structure, but the surface of the complex had a granular morphology. Caco-2 cell model tests revealed that the bioavailability of PPH21-Ca was 58.4 ± 0.5%, which was significantly higher than that of inorganic calcium at 37.0 ± 0.4%.
Peanut peptides can chelate calcium ions by carboxyl and amino groups, and the peptide-calcium complex had higher bioavailability. This study provides a theoretical basis for the development of new calcium supplement products that are absorbed easily. © 2024 Society of Chemical Industry.
花生肽与金属离子具有良好的螯合能力。然而，关于花生肽与钙的螯合机制以及肽 - 钙复合物的吸收特性的研究较少。
从花生蛋白水解物（PPH）中分离纯化出具有高钙螯合率的肽，组分F21的螯合率较高（81.4±0.8%）。通过液相色谱 - 串联质谱从组分F21中鉴定出六种肽，这六种肽的氨基酸序列中酸性氨基酸和精氨酸的出现频率均较高。选择组分F21（PPH21）制备花生肽钙复合物（PPH21 - Ca）。紫外分析表明花生肽与钙离子之间发生了螯合反应。傅里叶变换红外分析表明螯合位点是肽氨基酸残基上的羧基和氨基。扫描电子显微镜显示花生肽表面具有光滑的块状结构，但复合物表面具有颗粒形态。Caco - 2细胞模型试验表明PPH21 - Ca的生物利用度为58.4±0.5%，显著高于无机钙的37.0±0.4%。
花生肽可通过羧基和氨基螯合钙离子，且肽 - 钙复合物具有较高的生物利用度。本研究为开发易于吸收的新型补钙产品提供了理论依据。©2024化学工业协会。
