Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, China.
Key Laboratory of Vegetation Ecology, Ministry of Education, Northeast Normal University, Changchun 130024, China.
J Agric Food Chem. 2024 Apr 10;72(14):7735-7748. doi: 10.1021/acs.jafc.3c09095. Epub 2024 Mar 28.
Reverse chemical ecology has been widely applied for the functional characterization of olfactory proteins in various arthropods, but few related studies have focused on parasitic wasps. Here, the odorant carrier Niemann-Pick C2 protein of (BdioNPC2b) was studied in vitro and in vivo. Ligand binding analysis revealed that BdioNPC2b most strongly bound to 2-butyl-2-octenal and which compound could elicit an EAG response and attracted adults. Moreover, this odorant attractant significantly improved the reproductive efficiency of compared to that of the control. Then, the relationship between BdioNPC2b and 2-butyl-2-octenal was validated by RNAi, and site-directed mutagenesis revealed the involvement of three key residues of BdioNPC2b in binding to 2-butyl-2-octenal through hydrogen bonding. Our findings provide not only a deeper understanding of the olfactory function of NPC2 in wasps but also useful information for improving the performance of the parasitoid as a biological control agent.
反转化学生态学已被广泛应用于各种节肢动物嗅觉蛋白的功能表征，但很少有相关研究集中在寄生蜂上。在这里，研究了 （BdioNPC2b）的气味载体 Niemann-Pick C2 蛋白的体外和体内情况。配体结合分析表明，BdioNPC2b 与 2-丁基-2-辛烯醛结合最强，而该化合物可以引起 EAG 反应并吸引 成虫。此外，与对照相比，这种气味引诱剂显著提高了 的繁殖效率。然后，通过 RNAi 验证了 BdioNPC2b 与 2-丁基-2-辛烯醛之间的关系，定点突变揭示了 BdioNPC2b 中三个关键残基通过氢键参与与 2-丁基-2-辛烯醛的结合。我们的研究结果不仅提供了对 NPC2 在蜂类中的嗅觉功能的更深入理解，也为提高寄生蜂作为生物防治剂的性能提供了有用信息。
