Department of Life Science, Acharya Narendra Dev College, University of Delhi, Kalkaji, New Delhi, India.
Faculty of Biological Sciences, Friedrich Schiller University, Jena, Germany.
J Basic Microbiol. 2024 Jun;64(6):e2400027. doi: 10.1002/jobm.202400027. Epub 2024 Mar 28.
Bacteriophages infecting Mycobacterium smegmatis mc155 are numerous and, hence, are classified into clusters based on nucleotide sequence similarity. Analyzing phages belonging to clusters/subclusters can help gain deeper insights into their biological features and potential therapeutic applications. In this study, for genomic characterization of B1 subcluster mycobacteriophages, a framework of online tools was developed, which enabled functional annotation of about 55% of the previously deemed hypothetical proteins in B1 phages. We also studied the phenotype, lysogeny status, and antimycobacterial activity of 10 B1 phages against biofilm and an antibiotic-resistant M. smegmatis strain (4XR1). All 10 phages belonged to the Siphoviridae family, appeared temperate based on their spontaneous release from the putative lysogens and showed antibiofilm activity. The highest inhibitory and disruptive effects on biofilm were 64% and 46%, respectively. This systematic characterization using a combination of genomic and experimental tools is a promising approach to furthering our understanding of viral dark matter.
感染耻垢分枝杆菌 mc155 的噬菌体数量众多，因此根据核苷酸序列的相似性分为聚类。分析属于聚类/亚聚类的噬菌体有助于更深入地了解它们的生物学特征和潜在的治疗应用。在这项研究中，为了对 B1 亚群分枝杆菌噬菌体进行基因组特征分析，开发了一个在线工具框架，该框架能够对 B1 噬菌体中约 55%以前被认为是假设的蛋白质进行功能注释。我们还研究了 10 种 B1 噬菌体针对生物膜和抗药性耻垢分枝杆菌菌株 (4XR1) 的表型、溶原状态和抗分枝杆菌活性。所有 10 种噬菌体都属于肌尾噬菌体科，根据其从推定的溶原菌中的自发释放，表现出温和噬菌体的特征，并显示出抗生物膜活性。对生物膜的最高抑制和破坏作用分别为 64%和 46%。使用基因组和实验工具的组合进行这种系统的特征分析，是进一步了解病毒暗物质的一种很有前途的方法。
