RIKEN Brain Science Institute, Wako, Saitama, 351-0198, Japan.
Department Physiology and Cell Biology, Kobe University School of Medicine, Chuo, Kobe, 650-0117, Japan.
Nat Commun. 2024 Mar 28;15(1):2496. doi: 10.1038/s41467-024-46529-9.
Postsynaptic proteins play crucial roles in synaptic function and plasticity. During brain development, alterations in synaptic number, shape, and stability occur, known as synapse maturation. However, the postsynaptic protein composition changes during development are not fully understood. Here, we show the trajectory of the postsynaptic proteome in developing male mice and common marmosets. Proteomic analysis of mice at 2, 3, 6, and 12 weeks of age shows that proteins involved in synaptogenesis are differentially expressed during this period. Analysis of published transcriptome datasets shows that the changes in postsynaptic protein composition in the mouse brain after 2 weeks of age correlate with gene expression changes. Proteomic analysis of marmosets at 0, 2, 3, 6, and 24 months of age show that the changes in the marmoset brain can be categorized into two parts: the first 2 months and after that. The changes observed in the first 2 months are similar to those in the mouse brain between 2 and 12 weeks of age. The changes observed in marmoset after 2 months old include differential expression of synaptogenesis-related molecules, which hardly overlap with that in mice. Our results provide a comprehensive proteomic resource that underlies developmental synapse maturation in rodents and primates.
突触后蛋白在突触功能和可塑性中发挥着关键作用。在大脑发育过程中，突触数量、形状和稳定性发生改变，这被称为突触成熟。然而，发育过程中突触后蛋白组成的变化还不完全清楚。在这里，我们展示了雄性小鼠和普通狨猴发育过程中突触后蛋白组的轨迹。对 2、3、6 和 12 周龄小鼠的蛋白质组学分析表明，在此期间，参与突触发生的蛋白质表达存在差异。对已发表的转录组数据集的分析表明，2 周龄后小鼠大脑中突触后蛋白组成的变化与基因表达变化相关。对 0、2、3、6 和 24 月龄狨猴的蛋白质组学分析表明，狨猴大脑的变化可以分为两部分：前 2 个月和之后。前 2 个月观察到的变化与小鼠 2 周至 12 周龄之间的大脑变化相似。2 个月后观察到的狨猴变化包括与突触发生相关的分子的差异表达，这些变化与小鼠的变化几乎没有重叠。我们的研究结果提供了一个全面的蛋白质组资源，为啮齿动物和灵长类动物的发育性突触成熟提供了基础。
