Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Hermilio Valdizan, Huánuco, Peru.
Laboratorio de Biotecnología Molecular, Unidad Central de Laboratorios, Universidad Nacional Hermilio Valdizan, Huánuco, Peru.
Open Vet J. 2024 Feb;14(2):716-729. doi: 10.5455/OVJ.2024.v14.i2.12. Epub 2024 Feb 29.
Presently, there exists a growing interest in mitigating the utilization of antibiotics in response to the challenges emanating from their usage in livestock. A viable alternative strategy encompasses the introduction of live microorganisms recognized as probiotics, exerting advantageous impacts on the immune system and nutritional aspects of the host animals. Native lactic acid bacteria, inherently possessing specific properties and adaptive capabilities tailored to each animal, are deemed optimal contenders for probiotic advancement.
In the current investigation, microorganisms exhibiting probiotic potential were isolated, characterized, and identified from the fecal samples of guinea pigs () belonging to the Peruvian breed.
The lactic acid bacteria isolated on Man, Rogosa, and Sharpe agar underwent Gram staining, catalase testing, proteolytic, amylolytic, and cellulolytic activity assays, low pH tolerance assessment, hemolytic evaluation, antagonism against ., determination of autoaggregation and coaggregation capacity, and genotypic characterization through sequencing of the 16S rRNA gene.
A total of 33 lactic acid bacteria were isolated from the feces of 30 guinea pigs, also 10 isolates were selected based on Gram staining and catalase testing. All strains exhibited proteolytic activity, while only one demonstrated amylolytic capability, and none displayed cellulase activity. These bacteria showed higher tolerance to pH 5.0 and, to a lesser extent, to pH 4.0. Furthermore, they exhibited antagonistic activity against . Only two bacteria demonstrated hemolytic activity, and were subsequently excluded from further evaluations. Subsequent assessments revealed autoaggregation capacities ranging from 4.55% to 23.19%, with a lesser degree of coaggregation with . ranging from 3.53% to 8.94% for the remaining eight bacterial isolates. Based on these comprehensive tests, five bacteria with notable probiotic potential were identified by molecular assays as Leuconostoc citreum, and
The identified bacteria stand out as promising probiotic candidates, deserving further assessment in Peruvian breed guinea pigs. This exploration aims to enhance production outcomes while mitigating the adverse effects induced by pathogenic microorganisms.
目前，人们越来越关注减少抗生素的使用，以应对其在畜牧业中使用所带来的挑战。一种可行的替代策略是引入被认为是益生菌的活微生物，这些微生物对宿主动物的免疫系统和营养方面产生有益影响。天然乳酸菌具有针对每种动物的特定特性和适应能力，被认为是益生菌发展的最佳候选者。
在本研究中，从属于秘鲁品种的豚鼠粪便中分离、鉴定和表征具有益生菌潜力的微生物。
在 Man、Rogosa 和 Sharpe 琼脂上分离出的乳酸菌进行革兰氏染色、过氧化氢酶试验、蛋白水解、淀粉水解、纤维素水解活性测定、低 pH 值耐受评估、溶血评价、拮抗作用、自聚集和共聚能力的测定，以及通过 16S rRNA 基因测序进行基因型特征分析。
从 30 只豚鼠的粪便中分离出 33 株乳酸菌，根据革兰氏染色和过氧化氢酶试验选择了 10 株菌。所有菌株均表现出蛋白水解活性，只有一株表现出淀粉水解能力，没有一株表现出纤维素酶活性。这些细菌对 pH 5.0 的耐受性较高，对 pH 4.0 的耐受性较低。此外，它们对 具有拮抗作用。只有两株菌具有溶血活性，因此被排除在进一步评价之外。进一步评估显示，自聚集能力范围为 4.55%至 23.19%，与 之间的共聚能力范围为 3.53%至 8.94%，对于其余 8 株细菌分离株。基于这些综合测试，通过分子分析鉴定出 5 株具有显著益生菌潜力的细菌，分别为 Leuconostoc citreum、和
鉴定出的细菌是有前途的益生菌候选者，值得在秘鲁品种豚鼠中进一步评估。本研究旨在提高生产效益，同时减轻致病性微生物带来的不利影响。
