Yan Yusi, Xia Haiyang
Institute of Biopharmaceuticals, Taizhou University, Taizhou, China.
NHC Key Lab of Reproduction Regulation (Shanghai Institute for Biomedical and Pharmaceutical Technologies), School of Public Health, Fudan University, Shanghai, China.
Front Microbiol. 2024 Mar 14;15:1368809. doi: 10.3389/fmicb.2024.1368809. eCollection 2024.
species are best known for their ability to produce abundant secondary metabolites with versatile bioactivities and industrial importance. These metabolites are usually biosynthesized through metabolic pathways encoded by cluster-situated genes. These genes are also known as biosynthetic gene clusters (BGCs) of secondary metabolites. The expression of BGCs is intricately controlled by pyramidal transcriptional regulatory cascades, which include various regulators. antibiotic regulatory proteins (SARPs), a genus-specific family of regulators, are widely distributed and play important roles in regulating the biosynthesis of secondary metabolites in . Over the past decade, the biological functions of SARPs have been extensively investigated. Here, we summarized the recent advances in characterizing the roles of SARPs involved in secondary metabolism from the following three aspects. First, the classification and domain organization of SARPs were summarized according to their size variation. Second, we presented a detailed description of the regulatory mechanisms and modes of action of SARPs involved in secondary metabolism. Finally, the biotechnological application of SARPs was illustrated by improving the production of target secondary metabolites and discovering novel bioactive natural products. This review will help researchers to comprehensively understand the roles of SARPs in secondary metabolite biosynthesis in , which will contribute to building a solid foundation for their future application in synthetic biology.
物种以其产生具有多种生物活性和工业重要性的丰富次生代谢产物的能力而闻名。这些代谢产物通常通过由簇状基因编码的代谢途径进行生物合成。这些基因也被称为次生代谢产物的生物合成基因簇（BGCs）。BGCs的表达受到金字塔形转录调控级联的复杂控制，其中包括各种调控因子。抗生素调控蛋白（SARPs）是一类属特异性的调控因子家族，广泛分布并在调控[具体物种]中次生代谢产物的生物合成中发挥重要作用。在过去十年中，SARPs的生物学功能得到了广泛研究。在这里，我们从以下三个方面总结了表征参与[具体物种]次生代谢的SARPs作用的最新进展。首先，根据其大小变化总结了SARPs的分类和结构域组织。其次，我们详细描述了参与次生代谢的SARPs的调控机制和作用模式。最后，通过提高目标次生代谢产物的产量和发现新型生物活性天然产物来说明SARPs的生物技术应用。这篇综述将帮助研究人员全面了解SARPs在[具体物种]次生代谢产物生物合成中的作用，这将为它们未来在合成生物学中的应用奠定坚实基础。
