Central Research Laboratory, Institute of Medical Sciences and Sum Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, Odisha, 751003, India.
Department of Clinical Hematology, Institute of Medical Sciences and Sum Hospital, Siksha 'O' Anusandhan Deemed to be University, Bhubaneswar, Odisha, 751003, India.
Sci Rep. 2024 Mar 29;14(1):7513. doi: 10.1038/s41598-024-57972-5.
This research investigates the biogenic synthesis of silver nanoparticles (AgNPs) using the leaf extract of Chromolaena odorata (Asteraceae) and their potential as antibacterial and antifungal agents. Characterization techniques like ultraviolet-visible, Fourier transform infrared (FTIR), Dynamic light scattering and zeta potential (DLS), X-ray diffraction (XRD), transmission electron microscopy (TEM), and field emission scanning electron microscopy and energy-dispersive X-ray spectroscopy (FESEM-EDX) confirmed the formation of spherical (AgNPs). UV-vis spectroscopy reaffirms AgNP formation with a peak at 429 nm. DLS and zeta potential measurements revealed an average size of 30.77 nm and a negative surface charge (- 0.532 mV). Further, XRD analysis established the crystalline structure of the AgNPs. Moreover, the TEM descriptions indicate that the AgNPs are spherical shapes, and their sizes ranged from 9 to 22 nm with an average length of 15.27 nm. The X-ray photoelectron spectroscopy (XPS) analysis validated the formation of metallic silver and elucidated the surface state composition of AgNPs. Biologically, CO-AgNPs showed moderate antibacterial activity but excellent antifungal activity against Candida tropicalis (MCC 1559) and Trichophyton rubrum (MCC 1598). Low MIC values (0.195 and 0.390 mg/mL) respectively, suggest their potential as effective antifungal agents. This suggests potential applications in controlling fungal infections, which are often more challenging to treat than bacterial infections. Molecular docking results validated that bioactive compounds in C. odorata contribute to antifungal activity by interacting with its specific domain. Further research could pave the way for the development of novel and safe antifungal therapies based on biogenic nanoparticles.
本研究利用马缨丹（菊科）的叶提取物来合成生物银纳米粒子（AgNPs），并研究其作为抗菌和抗真菌剂的潜力。采用紫外-可见分光光度法、傅里叶变换红外光谱（FTIR）、动态光散射和zeta 电位（DLS）、X 射线衍射（XRD）、透射电子显微镜（TEM）、场发射扫描电子显微镜和能量色散 X 射线能谱（FESEM-EDX）等技术对 AgNPs 的形成进行了表征。紫外-可见光谱分析表明，AgNPs 的形成伴随着 429nm 处的峰值。DLS 和 zeta 电位测量结果表明，AgNPs 的平均粒径为 30.77nm，表面带负电荷（-0.532mV）。此外，XRD 分析确定了 AgNPs 的晶体结构。进一步的 TEM 描述表明，AgNPs 呈球形，粒径范围为 9-22nm，平均长度为 15.27nm。X 射线光电子能谱（XPS）分析证实了金属银的形成，并阐明了 AgNPs 的表面状态组成。生物实验表明，CO-AgNPs 对金黄色葡萄球菌（MCC 1559）和红色毛癣菌（MCC 1598）具有中等的抗菌活性，但对热带假丝酵母具有优异的抗真菌活性。较低的 MIC 值（分别为 0.195 和 0.390mg/mL）表明其作为有效抗真菌剂的潜力。这表明它们在控制真菌感染方面具有潜在应用，真菌感染的治疗通常比细菌感染更具挑战性。分子对接结果表明，马缨丹中的生物活性化合物通过与特定结构域相互作用，对其抗真菌活性起作用。进一步的研究可能为基于生物合成纳米粒子开发新型、安全的抗真菌疗法铺平道路。
