Boddé H E, Nelson D G, Koops P G, Arends J
J Dent Res. 1985 Jan;64(1):12-8. doi: 10.1177/00220345850640010101.
Initial lesions in bovine enamel were remineralized in vitro for periods lasting from one hour to two weeks; in some cases, remineralization was interrupted daily for a ten-minute APF application. After two weeks, surface coatings appeared on APF-treated specimens; SEM and TEM observations, including selected area and micro-electron diffraction, indicated both a layered structure within these coatings, and the predominance of calcium fluoride single crystals, ranging from 0.1 to 1.0 micrometer in size. Using double (45 Ca and 32 P) labeled remineralizing solutions, we obtained depth profiles of deposited labeled calcium and phosphate; these indicated that repeated APF applications prevented inward penetration of calcium and phosphate and limited the deposition of these ions to an outer surface region corresponding to the surface coating. These phenomena are explained in terms of the composition and apparent reactivity of the coating.
牛牙釉质的初始病变在体外进行再矿化处理，持续时间从1小时至2周不等；在某些情况下，每天中断再矿化处理10分钟以应用酸性磷酸氟（APF）。两周后，经APF处理的标本表面出现涂层；扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察，包括选区和微电子衍射，表明这些涂层内部具有层状结构，且主要是尺寸在0.1至1.0微米之间的氟化钙单晶。使用双标记（45钙和32磷）的再矿化溶液，我们获得了沉积的标记钙和磷的深度分布；这些结果表明，重复应用APF可阻止钙和磷向内渗透，并将这些离子的沉积限制在与表面涂层相对应的外表面区域。根据涂层的组成和表观反应性对这些现象进行了解释。
