School of Chemistry, Dalian University of Technology, Dalian, 116024, China.
School of Chemistry, Dalian University of Technology, Dalian, 116024, China.
Talanta. 2024 Jul 1;274:125982. doi: 10.1016/j.talanta.2024.125982. Epub 2024 Mar 27.
Hydrogen sulfide exhibits crucial functions in many biological and physiological processes. The abnormal levels of HS have been revealed to be associated with numerous human diseases. The majority of existing fluorescent probes toward HS may still need to be improved in terms of single output signal, water solubility, biotoxicity and photostability. The construction of a ratiometric fluorescent probe based on metal complex is one effective strategy for avoiding the mentioned problems for precisely detecting HS. Herein, we report an iridium(III) complex-based ratiometric luminescence probe (Ir-PNBD), which is designed by coupling the 7-nitro-2,1,3-benzoxadiazoles (NBD) to one of the bipyridine ligands of Ir (III) complex luminophore through a piperazition moiety. Ir-PNBD owns high selectivity and sensitivity toward HS, and an excellent ability to target mitochondria. Moreover, Ir-PNBD was further successfully utilized to visualize exogenous and endogenous HS in HeLa cells and zebrafish. Our work offers new opportunities to gain deeper insights into the construction of transition metal complex-based ratiometric luminescent probes and expands their applications in biomedical imaging and disease diagnosis.
硫化氢在许多生物和生理过程中发挥着关键作用。已经发现 HS 的异常水平与许多人类疾病有关。大多数现有的针对 HS 的荧光探针在单输出信号、水溶性、生物毒性和光稳定性方面仍需要改进。基于金属配合物构建比率荧光探针是避免上述问题以精确检测 HS 的一种有效策略。在这里，我们报告了一种基于铱(III)配合物的比率发光探针 (Ir-PNBD)，它是通过将 7-硝基-2,1,3-苯并恶二唑 (NBD) 通过哌嗪部分与 Ir (III) 配合物发光体的一个联吡啶配体偶联而设计的。Ir-PNBD 对 HS 具有高选择性和灵敏度，并且对线粒体具有优异的靶向能力。此外，Ir-PNBD 还成功地用于在 HeLa 细胞和斑马鱼中可视化外源性和内源性 HS。我们的工作为深入了解基于过渡金属配合物的比率发光探针的构建提供了新的机会，并扩展了它们在生物医学成像和疾病诊断中的应用。
