Institute of Plant Genetics of the Polish Academy of Sciences, Strzesynska 34, 60-479, Poznan, Poland.
Institute of Plant Genetics of the Polish Academy of Sciences, Strzesynska 34, 60-479, Poznan, Poland.
Chemosphere. 2024 May;356:141789. doi: 10.1016/j.chemosphere.2024.141789. Epub 2024 Mar 28.
Since nanoparticles (NPs) released into the environment from household or industrial wastes and applied directly on plants as agrochemicals can accumulate in the rhizosphere, it is imperative to understand how these NPs affect plant secondary metabolism upon their contact with the roots of intact plants. Here, the effects of Pd, Au, ZnO and FeO NPs on secondary metabolism were comprehensively investigated in Hypericum perforatum L float seedlings by analyzing 41 major secondary metabolites using ultra-performance liquid chromatography coupled with photodiode array, fluorescence detector and high-resolution mass spectrometry (UPLC-PDA-FLR-HRMS). The results showed that exposure of H. perforatum roots to Pd, Au, ZnO and FeO NPs rapidly led to fluctuations in the levels of secondary metabolites. Although these fluctuations did not correlate with NP type, concentration and duration of treatment, a total of 22 compounds were significantly altered by the NPs tested. In particular, 1 ppm Au increased the content of quercetin 3-(2″-acetylgalactoside), cadensin G and leutoskyrin by 5.02-, 2.12- and 2.58-fold, respectively after 24 h; 25 ppm Pd NPs led to a 2.1-fold increase in miquelianin content after 6 h; 50 ppm FeO NPs increased the level of furohyperforin by 3.09-fold and decreased the content of miquelianin 5.22-fold after 24 h and 50 ppm ZnO led to a 2.13-fold increase in hypericin after 48 h. These results emphasise the need to understand the intricate interplay between NPs and plant secondary metabolism in order to enable safer and efficient applications of NPs in agriculture.
由于家庭或工业废物中释放到环境中的纳米颗粒（NPs）以及作为农用化学品直接应用于植物时可以在根际中积累，因此了解这些 NPs 在与完整植物的根部接触时如何影响植物次生代谢是当务之急。在这里，通过使用超高效液相色谱-光电二极管阵列、荧光检测器和高分辨率质谱（UPLC-PDA-FLR-HRMS）分析 41 种主要次生代谢物，全面研究了 Pd、Au、ZnO 和 FeO NPs 对贯叶连翘浮苗次生代谢的影响。结果表明，Pd、Au、ZnO 和 FeO NPs 暴露于贯叶连翘根后，次生代谢物水平迅速波动。尽管这些波动与 NP 类型、处理浓度和时间无关，但总共 22 种化合物被测试的 NPs 显著改变。特别是，1 ppm Au 在 24 h 后分别使槲皮素 3-（2″-乙酰半乳糖苷）、cadensin G 和 leutoskyrin 的含量增加了 5.02、2.12 和 2.58 倍；25 ppm Pd NPs 在 6 h 后导致 miquelianin 含量增加 2.1 倍；50 ppm FeO NPs 在 24 h 后使 furohyperforin 的水平增加了 3.09 倍，miquelianin 的含量减少了 5.22 倍，50 ppm ZnO 在 48 h 后使 hypericin 的含量增加了 2.13 倍。这些结果强调了需要了解 NPs 与植物次生代谢之间的复杂相互作用，以便能够在农业中更安全、更有效地应用 NPs。
