Research institute for Fundamental sciences (RIFS), University of Tabriz, Tabriz, Iran.
Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Sci Rep. 2019 Sep 10;9(1):12998. doi: 10.1038/s41598-019-49504-3.
It appears that the biologically-synthesized nanoparticles (NPs) have potential to perform as effective elicitors for the production of valuable secondary metabolites in plants. Besides, it has been reported that the toxicity of the biologically-synthesized NP is not as much as that of the chemically-synthesized NPs. Therefore, it is necessary to test their advantages aspects. In this study, the physical synthesis of perlite NPs and biologically-synthesis of TiO/perlite nanocomposites (NCs) were conducted. Subsequently, their effects and explant source influence on the growth characteristics and secondary metabolite profiles of Hypericum perforatum callus cultures were evaluated. According to the obtained results, morphology of the synthesized perlite NPs and TiO/perlite NCs were mesoporous and spherical with sizes ranging about 14.51-23.34 and 15.50-24.61 nm, respectively. Addition of perlite NPs and TiO/perlite NCs to the culture medium at the concentration range of 25-200 mg/L showed no adverse impacts on the growth characteristics of H. perforatum calli. According to the GC-MS analysis, the stress caused by perlite NPs and TiO/perlite NCs led to an increase in the variety, amount and number of volatile compounds. The calli obtained from in vitro grown plants produced more volatile compounds relative to the calli obtained from field grown plants under the nanomaterial stress conditions. The production of hypericin and pseudohypericin were also determined in the callus cultures under desired nanomaterials elicitation. Accordingly, our results suggest that perlite NPs and TiO/perlite NCs can possibly be considered as effective elicitors for the production of volatile compounds, hypericin, and pseudohypericin in callus cultures of H. perforatum.
似乎生物合成的纳米粒子 (NPs) 具有作为植物中有价值的次生代谢产物的有效诱导剂的潜力。此外，据报道，生物合成的 NP 的毒性不如化学合成的 NP 那么大。因此，有必要测试它们的优势。在这项研究中，进行了珍珠岩 NPs 的物理合成和 TiO/珍珠岩纳米复合材料 (NCs) 的生物合成。随后，评估了它们的效果和外植体来源对贯叶连翘愈伤组织培养物生长特性和次生代谢产物谱的影响。根据获得的结果，合成的珍珠岩 NPs 和 TiO/珍珠岩 NCs 的形态为介孔和球形，尺寸分别约为 14.51-23.34nm 和 15.50-24.61nm。将珍珠岩 NPs 和 TiO/珍珠岩 NCs 添加到浓度范围为 25-200mg/L 的培养基中对贯叶连翘愈伤组织的生长特性没有不利影响。根据 GC-MS 分析，珍珠岩 NPs 和 TiO/珍珠岩 NCs 引起的应激导致挥发性化合物的种类、数量和数量增加。与纳米材料应激条件下田间生长的植物获得的愈伤组织相比，来自体外生长的植物的愈伤组织产生了更多的挥发性化合物。还在愈伤组织培养物中测定了贯叶连翘素和伪贯叶连翘素的产生。因此，我们的结果表明，珍珠岩 NPs 和 TiO/珍珠岩 NCs 可作为贯叶连翘愈伤组织中挥发性化合物、贯叶连翘素和伪贯叶连翘素生产的有效诱导剂。
